ZFIN ID: ZDB-FIG-131231-42
van der Velden et al., 2013 - The polycomb group protein ring1b/rnf2 is specifically required for craniofacial development. PLoS One   8(9):e73997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 6 Loss of endochondral and dermal ossification in ring1b mutants.

Ventral (A–D, E, F) and lateral (A′–D′, E′,) views of in situ hybridizations with riboprobes against the indicated genes in WT and ring1b mutants at 68–72 hpf. In WT embryos, runx2a and runx2b are expressed in hypertrophic pharyngeal arch-derived chondrocytes, as well as in the dermal ossification centers of the operculum, parasphenoid and cleithrum. Weak expression is detected in pharyngeal arches and the parasphenoid of ring1b mutants (B, B′, D, D′). At 72 hpf, col10a1 is expressed in developing dermal bones in WT embryos, but not in ring1b mutants (E–F). cl: cleithrum; de: dentary; h: hyoid; m: mandibular; mx: maxilla; pa: pharyngeal arches; pq: palatoquadrate; ps: parasphenoid, op: operculum cl: the cleithrum. Numbers indicate the respective pharyngeal arches.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
col10a1a WT standard conditions Protruding-mouth cleithrum ISH
Protruding-mouth opercle ISH
Protruding-mouth parasphenoid ISH
rnf2ibl30/ibl30 standard conditions Protruding-mouth Not Detected cleithrum ISH
Protruding-mouth Not Detected opercle ISH
Protruding-mouth Not Detected parasphenoid ISH
rnf2ibl31/ibl31 standard conditions Protruding-mouth Not Detected cleithrum ISH
Protruding-mouth Not Detected opercle ISH
Protruding-mouth Not Detected parasphenoid ISH
runx2a WT standard conditions Pec-fin cleithrum ISH
Pec-fin dentary ISH
Pec-fin maxilla ISH
Pec-fin opercle ISH
Pec-fin parasphenoid ISH
Pec-fin pharyngeal arch hypertrophic chondrocyte ISH
rnf2ibl30/ibl30 standard conditions Pec-fin dentary ISH
Pec-fin parasphenoid ISH
Pec-fin pharyngeal arch ISH
rnf2ibl31/ibl31 standard conditions Pec-fin dentary ISH
Pec-fin parasphenoid ISH
Pec-fin pharyngeal arch ISH
runx2b WT standard conditions Pec-fin branchiostegal ray ISH
Pec-fin cleithrum ISH
Pec-fin opercle ISH
Pec-fin parasphenoid ISH
Pec-fin pharyngeal arch hypertrophic chondrocyte ISH
rnf2ibl30/ibl30 standard conditions Pec-fin parasphenoid ISH
Pec-fin pharyngeal arch ISH
rnf2ibl31/ibl31 standard conditions Pec-fin parasphenoid ISH
Pec-fin pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rnf2ibl30/ibl30 standard conditions Protruding-mouth cleithrum poorly ossified, abnormal
Protruding-mouth direct ossification decreased process quality, abnormal
Protruding-mouth endochondral ossification decreased process quality, abnormal
Protruding-mouth opercle poorly ossified, abnormal
Protruding-mouth parasphenoid poorly ossified, abnormal
Protruding-mouth pharyngeal arch cartilage poorly ossified, abnormal
rnf2ibl31/ibl31 standard conditions Protruding-mouth cleithrum poorly ossified, abnormal
Protruding-mouth direct ossification decreased process quality, abnormal
Protruding-mouth endochondral ossification decreased process quality, abnormal
Protruding-mouth opercle poorly ossified, abnormal
Protruding-mouth parasphenoid poorly ossified, abnormal
Protruding-mouth pharyngeal arch cartilage poorly ossified, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One