ZFIN ID: ZDB-FIG-131231-39
van der Velden et al., 2013 - The polycomb group protein ring1b/rnf2 is specifically required for craniofacial development. PLoS One   8(9):e73997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Pec-fin

Fig. 3 Migration and initial differentiation of trunk neural crest is largely unaffected in ring1b mutants.

Sox10 staining of WT and ring1b mutants at 24 and 32 hpf shows that sox10 expressing NC cells have migrated timely and correctly to their ventral positions (A–D). Expression of foxD3 in cranial ganglia-associated glia is comparable to WT siblings in ring1b mutants at 32 hpf (E, F), but reduced in the postotic ganglia of ring1b mutants at 56 hpf (G, H). NeuroD expression in cranial ganglia precursors is reduced in ring1b mutants at 32 hpf (I, J), but remains detectable in the hindbrain region of ring1b mutants at 56 hpf (L). Lateral view of 32 hpf embryos shows normal distribution of melanocytes in ring1b mutants at 32 hpf (M, N). At 60 hpf, ring1b melanophores remain more stellate than WT melanophores, which have started to round up (S, T). Iridophores are present in near normal numbers in ring1b mutants at 60 hpf (O, P), whereas ring1b xanthophores are smaller and less stellate (Q, R, U, V). Abbreviations: gX: vagal ganglia; mll: medial lateral line ganglia; o/gVIII: octaval/statoacustic ganglia; pll/gP: posteriolateral line ganglia; pog: preotic ganglia; ptg: postotic ganglia; tg: trigeminal ganglia.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxd3 WT standard conditions Prim-15 trigeminal ganglion glial cell (sensu Vertebrata) ISH
Long-pec trigeminal ganglion glial cell (sensu Vertebrata) ISH
rnf2ibl30/ibl30 standard conditions Prim-15 trigeminal ganglion glial cell (sensu Vertebrata) ISH
Long-pec trigeminal ganglion glial cell (sensu Vertebrata) ISH
rnf2ibl31/ibl31 standard conditions Prim-15 trigeminal ganglion glial cell (sensu Vertebrata) ISH
Long-pec trigeminal ganglion glial cell (sensu Vertebrata) ISH
neurod1 WT standard conditions Prim-15 middle lateral line ganglion ISH
Prim-15 posterior lateral line ganglion ISH
Prim-15 statoacoustic (VIII) ganglion ISH
Prim-15 trigeminal ganglion ISH
Long-pec posterior lateral line ganglion ISH
Long-pec statoacoustic (VIII) ganglion ISH
Long-pec vagal ganglion ISH
rnf2ibl30/ibl30 standard conditions Prim-15 posterior lateral line ganglion ISH
Prim-15 statoacoustic (VIII) ganglion ISH
Prim-15 trigeminal ganglion ISH
Long-pec posterior lateral line ganglion ISH
Long-pec statoacoustic (VIII) ganglion ISH
Long-pec vagal ganglion ISH
rnf2ibl31/ibl31 standard conditions Prim-15 posterior lateral line ganglion ISH
Prim-15 statoacoustic (VIII) ganglion ISH
Prim-15 trigeminal ganglion ISH
Long-pec posterior lateral line ganglion ISH
Long-pec statoacoustic (VIII) ganglion ISH
Long-pec vagal ganglion ISH
sox10 WT standard conditions Prim-5 trunk neural crest ISH
Prim-15 trunk neural crest ISH
rnf2ibl30/ibl30 standard conditions Prim-5 trunk neural crest ISH
Prim-15 trunk neural crest ISH
rnf2ibl31/ibl31 standard conditions Prim-5 trunk neural crest ISH
Prim-15 trunk neural crest ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rnf2ibl30/ibl30 standard conditions Prim-5 neural crest cell migration process quality, normal
Prim-15 glial cell differentiation process quality, normal
Prim-15 melanocyte distributed, normal
Prim-15 neural crest cell migration process quality, normal
Long-pec neuron differentiation process quality, normal
Pec-fin iridophore normal amount, normal
Pec-fin melanocyte increased branchiness, abnormal
Pec-fin pigment cell differentiation process quality, normal
Pec-fin xanthophore decreased branchiness, abnormal
Pec-fin xanthophore decreased size, abnormal
rnf2ibl31/ibl31 standard conditions Prim-5 neural crest cell migration process quality, normal
Prim-15 glial cell differentiation process quality, normal
Prim-15 melanocyte distributed, normal
Prim-15 neural crest cell migration process quality, normal
Long-pec neuron differentiation process quality, normal
Pec-fin iridophore normal amount, normal
Pec-fin melanocyte increased branchiness, abnormal
Pec-fin pigment cell differentiation process quality, normal
Pec-fin xanthophore decreased branchiness, abnormal
Pec-fin xanthophore decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One