ZFIN ID: ZDB-FIG-131231-41
van der Velden et al., 2013 - The polycomb group protein ring1b/rnf2 is specifically required for craniofacial development. PLoS One   8(9):e73997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 5 Impaired formation of prechondrogenic condensations and abolished cartilage differentiation in ring1b mutants.

Ventral (A–J) and lateral (A′–J′) views of WT and ring1b mutants at 50 hpf stained with the indicated genes. Prechondrogenic condensation markers sox9a and sox9b are expressed in ring1b CNCs residing in pharyngeal arches 1 and 2, albeit the expression domain is smaller (A–D′). Similarly, the expression domain of CNC markers sox10 and dlx2a is reduced in ring1b pharyngeal arches 1 and 2 (E–H′). Expression of sox9a, sox9b and sox10 is not detected at the presumptive location of the trabeculae in ring1b mutants (B, B′, D, D′, F, F′). Col2a1 is expressed in WT trabeculae and pharyngeal arches I–II, but expression is abolished in ring1b mutants (I–, J′). Note that expression of col2a1, a marker for differentiating chondrocytes, is normal in the otic vesicles and parachordals of ring1b mutants. tc: trabeculae cranii; pc: parachordal; ov: otic vesicle. Numbers indicate the respective pharyngeal arches.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
col2a1a WT standard conditions Long-pec neurocranial trabecula chondrocyte ISH
Long-pec otic vesicle chondrocyte ISH
Long-pec pharyngeal arch 1 chondrocyte ISH
Long-pec pharyngeal arch 2 chondrocyte ISH
rnf2ibl30/ibl30 standard conditions Long-pec Not Detected neurocranial trabecula chondrocyte ISH
Long-pec otic vesicle chondrocyte ISH
Long-pec parachordal cartilage chondrocyte ISH
Long-pec Not Detected pharyngeal arch 1 chondrocyte ISH
Long-pec Not Detected pharyngeal arch 2 chondrocyte ISH
rnf2ibl31/ibl31 standard conditions Long-pec Not Detected neurocranial trabecula chondrocyte ISH
Long-pec otic vesicle chondrocyte ISH
Long-pec parachordal cartilage chondrocyte ISH
Long-pec Not Detected pharyngeal arch 1 chondrocyte ISH
Long-pec Not Detected pharyngeal arch 2 chondrocyte ISH
dlx2a WT standard conditions Long-pec neurocranial trabecula cranial neural crest cell ISH
Long-pec pharyngeal arch 1 cranial neural crest cell ISH
Long-pec pharyngeal arch 2 cranial neural crest cell ISH
rnf2ibl30/ibl30 standard conditions Long-pec Not Detected neurocranial trabecula cranial neural crest cell ISH
Long-pec pharyngeal arch 1 cranial neural crest cell ISH
Long-pec pharyngeal arch 2 cranial neural crest cell ISH
rnf2ibl31/ibl31 standard conditions Long-pec Not Detected neurocranial trabecula cranial neural crest cell ISH
Long-pec pharyngeal arch 1 cranial neural crest cell ISH
Long-pec pharyngeal arch 2 cranial neural crest cell ISH
sox9a WT standard conditions Long-pec neurocranial trabecula chondroblast ISH
Long-pec pharyngeal arch 1 chondroblast ISH
Long-pec pharyngeal arch 2 chondroblast ISH
Long-pec pharyngeal arch 3-7 chondroblast ISH
rnf2ibl30/ibl30 standard conditions Long-pec Not Detected neurocranial trabecula chondroblast ISH
Long-pec pharyngeal arch 1 chondroblast ISH
Long-pec pharyngeal arch 2 chondroblast ISH
rnf2ibl31/ibl31 standard conditions Long-pec Not Detected neurocranial trabecula chondroblast ISH
Long-pec pharyngeal arch 1 chondroblast ISH
Long-pec pharyngeal arch 2 chondroblast ISH
sox9b WT standard conditions Long-pec neurocranial trabecula chondroblast ISH
Long-pec pharyngeal arch 1 chondroblast ISH
Long-pec pharyngeal arch 2 chondroblast ISH
Long-pec pharyngeal arch 3-7 chondroblast ISH
rnf2ibl30/ibl30 standard conditions Long-pec Not Detected neurocranial trabecula chondroblast ISH
Long-pec pharyngeal arch 1 chondroblast ISH
Long-pec pharyngeal arch 2 chondroblast ISH
Long-pec Not Detected pharyngeal arch 3-7 chondroblast ISH
rnf2ibl31/ibl31 standard conditions Long-pec Not Detected neurocranial trabecula chondroblast ISH
Long-pec pharyngeal arch 1 chondroblast ISH
Long-pec pharyngeal arch 2 chondroblast ISH
Long-pec Not Detected pharyngeal arch 3-7 chondroblast ISH
sox10 WT standard conditions Long-pec neurocranial trabecula cranial neural crest cell ISH
Long-pec pharyngeal arch 1 cranial neural crest cell ISH
Long-pec pharyngeal arch 2 cranial neural crest cell ISH
rnf2ibl30/ibl30 standard conditions Long-pec Not Detected neurocranial trabecula cranial neural crest cell ISH
Long-pec pharyngeal arch 1 cranial neural crest cell ISH
Long-pec pharyngeal arch 2 cranial neural crest cell ISH
rnf2ibl31/ibl31 standard conditions Long-pec Not Detected neurocranial trabecula cranial neural crest cell ISH
Long-pec pharyngeal arch 1 cranial neural crest cell ISH
Long-pec pharyngeal arch 2 cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rnf2ibl30/ibl30 standard conditions Long-pec chondrocyte differentiation decreased process quality, abnormal
Long-pec neurocranial trabecula chondrocyte undifferentiated, abnormal
Long-pec pharyngeal arch chondrocyte undifferentiated, abnormal
rnf2ibl31/ibl31 standard conditions Long-pec chondrocyte differentiation decreased process quality, abnormal
Long-pec neurocranial trabecula chondrocyte undifferentiated, abnormal
Long-pec pharyngeal arch chondrocyte undifferentiated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One