FIGURE

Fig. 11

ID

ZDB-FIG-131029-40

Publication

Novikov et al., 2013 - Tmem88a mediates GATA-dependent specification of cardiomyocyte progenitors by restricting WNT signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 11

Limited WNT signaling sensitizes cardiogenic mesoderm to subthreshold depletion of Tmem88a. (A-D) WISH for nkx2.5 (box) and ntl in representative 8-somite stage control morphants (Ctrl) and subthreshold tmem88a morphants (88a), without or with (+wnt8) low-level WNT activation. Retraction of the notochord and general widening of the mesodermal plate occur in heat-shocked embryos, but independently of MO injection. Heat-shock treatment was administered to 75% epiboly embryos for 30 minutes. n>20 embryos analyzed per panel. (E) nkx2.5⁺ area normalized to control values. n=10 embryos measured per condition. (F) nkx2.5 relative expression levels assessed by qPCR. ^*P<0.05, ^**P<0.01; ns, not significant; error bars indicate s.d.

Expression Data

Genes:	nkx2.5 tbxta
Fish:	w34Tg w34Tg + MO3-tmem88a + MO4-tmem88a
Condition:	heat shock
Knockdown Reagents:	MO3-tmem88a MO4-tmem88a
Anatomical Terms:	myocardial precursor notochord
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w34Tg w34Tg + MO3-tmem88a + MO4-tmem88a
Condition:	heat shock
Knockdown Reagents:	MO3-tmem88a MO4-tmem88a
Observed In:	canonical Wnt signaling pathway cardioblast cell fate specification myocardial precursor
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development