FIGURE

Fig. S3

ID

ZDB-FIG-131029-44

Publication

Novikov et al., 2013 - Tmem88a mediates GATA-dependent specification of cardiomyocyte progenitors by restricting WNT signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

Design and validation of tmem88a morpholinos. (A) Morpholino design. A translation blocker (tbMO) was targeted to the translation start site (atg) of tmem88a mRNA. A splice-site blocker (ssMO) was targeted to the boundary between intron 2 (ii) and exon 3 (3) of the mRNA. (B) The efficacy of the ssMO was monitored by semi-quantitative reverse-transcription PCR. The abundance of the normal tmem88a transcript (196 bp) was reduced with optimal doses of ssMO. An unspliced transcript (2890 bp) accumulated that is predicted to encode a premature stop codon (UGA), confirmed by sequencing. Levels of β-actin were used as a loading control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development