FIGURE

Fig. 8

ID

ZDB-FIG-131029-38

Publication

Novikov et al., 2013 - Tmem88a mediates GATA-dependent specification of cardiomyocyte progenitors by restricting WNT signaling

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Fig. 8

Loss of CPs in tmem88a morphants is not caused by increased apoptosis, decreased cell proliferation or fate conversion to the hematovascular or forelimb lineages. Dorsal views of representative flatmount preparations of 8-somite (A-H) and 23-somite (I,J) control and tmem88a morphants, with anterior to the top (n>20 embryos analyzed per condition). (A,B) WISH for nkx2.5 and ntl, showing the cardiogenic mesoderm (arrowheads). (C,D) Apoptosis detection by TMR-Red. (E,F) Phospho-Histone H3 antibody staining for cell proliferation. (G,H) WISH for the hematovascular marker scl and for ntl. Arrow indicates the boundary of the anterior scl expression domain. (I,J) WISH for the forelimb marker tbx5.

Expression Data

Genes:	nkx2.5 tal1 tbx5a tbxta
Fish:	WT WT + MO3-tmem88a + MO4-tmem88a
Knockdown Reagents:	MO3-tmem88a MO4-tmem88a
Anatomical Terms:	myocardial precursor notochord pectoral fin field rostral blood island
Stage Range:	5-9 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-tmem88a + MO4-tmem88a
Knockdown Reagents:	MO3-tmem88a MO4-tmem88a
Observed In:	anterior lateral plate mesoderm apoptotic process anterior lateral plate mesoderm mitotic cell cycle blood island pectoral fin field
Stage Range:	5-9 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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