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General Information
ZFIN ID: ZDB-FIG-131029-34
Novikov et al., 2013 - Tmem88a mediates GATA-dependent specification of cardiomyocyte progenitors by restricting WNT signaling. Development (Cambridge, England)   140(18):3787-98 Full text @ Development
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

Loss of Tmem88a results in profound cardiomyopathy. (A) Brightfield images of representative control (MOCtrl) and tmem88a (MOT88a) morphants at 48 hpf. Arrowhead indicates pericardial edema. (B,C) Higher magnification of craniocardiac tissues in control and tmem88a morphants. Brain (br), eye (e), yolk (y), heart (hrt, arrowheads) and blood (bl, arrow) are indicated. (D,E) Fluorescent images of control and tmem88a morphant hearts observed in the tg(myl7:eGFP) background. Ventricle (v) and atrium (a) are indicated. n>50 embryos analyzed per panel. Scale bars: 0.1 mm in B-E.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
twu34Tg + MO3-tmem88a + MO4-tmem88a standard conditions Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-tmem88a + MO4-tmem88a standard conditions Long-pec cranial cartilage decreased object quality, abnormal
Long-pec embryonic hemopoiesis decreased process quality, abnormal
Long-pec heart edematous, abnormal
Long-pec heart contraction decreased frequency, abnormal
Long-pec trunk decreased length, abnormal
Long-pec whole organism lacks all parts of type blood, abnormal
twu34Tg + MO3-tmem88a + MO4-tmem88a standard conditions Protruding-mouth heart morphology, abnormal
Protruding-mouth heart looping decreased process quality, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development