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Fig. 11
Limited WNT signaling sensitizes cardiogenic mesoderm to subthreshold depletion of Tmem88a. (A-D) WISH for nkx2.5 (box) and ntl in representative 8-somite stage control morphants (Ctrl) and subthreshold tmem88a morphants (88a), without or with (+wnt8) low-level WNT activation. Retraction of the notochord and general widening of the mesodermal plate occur in heat-shocked embryos, but independently of MO injection. Heat-shock treatment was administered to 75% epiboly embryos for 30 minutes. n>20 embryos analyzed per panel. (E) nkx2.5+ area normalized to control values. n=10 embryos measured per condition. (F) nkx2.5 relative expression levels assessed by qPCR. *P<0.05, **P<0.01; ns, not significant; error bars indicate s.d.