FIGURE

Fig. 2

ID

ZDB-FIG-131029-32

Publication

Novikov et al., 2013 - Tmem88a mediates GATA-dependent specification of cardiomyocyte progenitors by restricting WNT signaling

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Fig. 2

Confirmation of gata5/6-dependent tmem88a expression in the anterior lateral plate mesoderm. (A,B) RNA sequencing tmem88a expression in RPKM (A) and qPCR tmem88a relative expression (B) for wild-type (black) and gata5/6 morphants (white) at bud and 6-somite (6ss) stages of development. (C,D) Transcript localization of tmem88a and the regional notochord (nt) marker ntl, shown in flatmount in situ hybridization preparations of 6-somite stage control (C) and gata5/6 (D) morphants (dorsal views, anterior to the left). Insets are higher magnifications of the boxed regions. tmem88a expression in the anterior lateral plate mesoderm is outlined (dotted line). n>20 embryos analyzed per panel. ^*P<0.05, ^**P<0.01; error bars indicate s.d.

Expression Data

Genes:	tbxta tmem88a
Fish:	WT WT + MO1-gata6 + MO2-gata5
Knockdown Reagents:	MO1-gata6 MO2-gata5
Anatomical Terms:	anterior lateral plate mesoderm notochord whole organism
Stage Range:	Bud to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gata6 + MO2-gata5
Knockdown Reagents:	MO1-gata6 MO2-gata5
Observed In:	anterior lateral plate mesoderm
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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