ZFIN ID: ZDB-FIG-121026-29
Tanaka et al., 2012 - Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish. Developmental Biology   370(2):223-236 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. 5 Loss of function of cdk5, dyrk2, dpysl2, and dpysl3 specifically affected the patterning of the dorsal-most part of the spinal cord. (A–L) Expression of nkx6.1 (A–C), iro3 (D–F), lim1 (G–I), and wnt1 (J–L) in the wild-type embryos (A, D, G, J), the dpysl2 and dpysl3 double morphants (B, E, H, K), and the cdk5 and dyrk2 double morphants (C, F, I, L) at 28 hpf, anterior left (A–I), anterior top (J–L). In the wild-type embryos, nkx6.1, iro3, and lim1 were expressed in the ventral, middle to dorsal, and dorsal part of the spinal cord, respectively (A, D, G). In the dorsal-most part of the spinal cord, wnt1 was expressed around the midline (J). In the dpysl2 and dpysl3 double morphants or the cdk5 and dyrk2 double morphants, the expression patterns of nkx6.1, iro3, and lim1 were similar to those of the wild-type embryos (B, C, E, F, H, I). However, the expression domain of wnt1 was abnormally distributed around the midline (K, L). Brackets in A–I and broken lines in J–L indicate the spinal cord.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
irx3a WT standard conditions Prim-5 spinal cord dorsal region ISH
Prim-5 spinal cord medial region ISH
WT + MO1-cdk5 + MO1-dyrk2 standard conditions Prim-5 spinal cord dorsal region ISH
Prim-5 spinal cord medial region ISH
WT + MO1-dpysl3 + MO3-dpysl2b standard conditions Prim-5 spinal cord dorsal region ISH
Prim-5 spinal cord medial region ISH
lhx1a WT standard conditions Prim-5 interneuron ISH
Prim-5 spinal cord dorsal region ISH
WT + MO1-cdk5 + MO1-dyrk2 standard conditions Prim-5 interneuron ISH
Prim-5 spinal cord dorsal region ISH
WT + MO1-dpysl3 + MO3-dpysl2b standard conditions Prim-5 interneuron ISH
Prim-5 spinal cord dorsal region ISH
nkx6.1 WT standard conditions Prim-5 spinal cord ventral region ISH
WT + MO1-cdk5 + MO1-dyrk2 standard conditions Prim-5 spinal cord ventral region ISH
WT + MO1-dpysl3 + MO3-dpysl2b standard conditions Prim-5 spinal cord ventral region ISH
wnt1 WT standard conditions Prim-5 spinal cord dorsal-most region ISH
WT + MO1-cdk5 + MO1-dyrk2 standard conditions Prim-5 spinal cord medial region ISH
WT + MO1-dpysl3 + MO3-dpysl2b standard conditions Prim-5 spinal cord medial region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 370(2), Tanaka, H., Morimura, R., and Ohshima, T., Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish, 223-236, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.