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ZFIN ID: ZDB-FIG-121026-27
Tanaka et al., 2012 - Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish. Developmental Biology   370(2):223-236 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3 Loss of function of cdk5 and dyrk2 inhibited the proper positioning of Rohon-Beard neuron in the dorsal part of the spinal cord. (A–D) Gene expression pattern of cdk5 (A–C) and dyrk2 (D–F) at 12 hpf (A, B, D, E) and at 28 hpf (C, F). Lateral view, anterior left (A, D) and dorsal view, anterior top (B, C, E, F). (G–I) The position of RB neurons in the dorsal spinal cord of each morphant at 28 hpf. Dorsal view, anterior top. In the morphants, the position of RB neurons shifted medially compare to the wild-type embryos (see Fig. 1I). (J, K) Quantitative data for the percentage of RB neurons on the midline (J), and total nomber of RB neurons within 3 segments (K). Data are mean ± SEM; NNNP<0.001, different from the wild-type embryos using 2-tailed paired Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdk5 WT standard conditions 5-9 somites central nervous system ISH
Prim-5 spinal cord medial-lateral axis ISH
dyrk2 WT standard conditions 5-9 somites central nervous system ISH
Prim-5 spinal cord medial-lateral axis ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdk5 standard conditions Prim-5 Rohon-Beard neuron displaced to spinal cord axis, abnormal
WT + MO1-cdk5 + MO1-dyrk2 standard conditions Prim-5 Rohon-Beard neuron displaced to spinal cord axis, abnormal
WT + MO1-dyrk2 standard conditions Prim-5 Rohon-Beard neuron displaced to spinal cord axis, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 370(2), Tanaka, H., Morimura, R., and Ohshima, T., Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish, 223-236, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.