Gene
nkx6.1
- ID
- ZDB-GENE-040718-178
- Name
- NK6 homeobox 1
- Symbol
- nkx6.1 Nomenclature History
- Previous Names
-
- zgc:92879
- Type
- protein_coding_gene
- Location
- Chr: 21 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within cell fate commitment; endocrine pancreas development; and spinal cord motor neuron differentiation. Predicted to be active in nucleus. Is expressed in several structures, including DEL; endoderm; nervous system; neural tube; and pancreatic system. Orthologous to human NKX6-1 (NK6 homeobox 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 44 figures from 31 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| ki130Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR | |
| ki131Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR | |
| ki132Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR | |
| sa3108 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| ulg005 | Allele with one deletion | Unknown | Frameshift, Premature Stop | CRISPR | |
| um321 | Allele with one deletion | Exon 1 | Frameshift, Premature Stop | CRISPR | |
| um322 | Allele with one delins | Exon 1 | Frameshift, Premature Stop | CRISPR |
1 - 7 of 7
Show
| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-nkx6.1 | Ghaye et al., 2015 | |
| CRISPR2-nkx6.1 | Yildiz et al., 2019 | |
| CRISPR3-nkx6.1 | (2) | |
| MO1-nkx6.1 | N/A | (5) |
| MO2-nkx6.1 | N/A | Binot et al., 2010 |
1 - 5 of 5
Show
Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Length | Helix-turn-helix motif | Homedomain-like superfamily | Homeobox, conserved site | Homeobox NK-like transcription regulators | Homeodomain | Homeodomain, metazoa |
|---|---|---|---|---|---|---|---|
|
UniProtKB:Q6DGK5
|
312 |
1 - 1 of 1
| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
nkx6.1-201
(1)
|
Ensembl | 2,023 nt |
1 - 1 of 1
Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| TgBAC(nkx6.1:EGFP) |
|
| 1 | (5) |
1 - 1 of 1
Show
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-203L7 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:92879 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:191271 | ZFIN Curated Data |
1 - 3 of 3
Show
| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001002475 (1) | 2045 nt | ||
| Genomic | GenBank:BX000991 (2) | 170966 nt | ||
| Polypeptide | UniProtKB:Q6DGK5 (1) | 312 aa |
- Mi, J., Andersson, O. (2023) Efficient knock-in method enabling lineage tracing in zebrafish. Life science alliance. 6(5)
- Mi, J., Liu, K.C., Andersson, O. (2023) Decoding pancreatic endocrine cell differentiation and β cell regeneration in zebrafish. Science advances. 9:eadf5142
- Chen, Y., Jia, J., Zhao, Q., Zhang, Y., Huang, B., Wang, L., Tian, J., Huang, C., Li, M., Li, X. (2022) Novel Loss-of-Function Variant in HNF1a Induces β-Cell Dysfunction through Endoplasmic Reticulum Stress. International Journal of Molecular Sciences. 23(21):
- Tschaikner, P., Regele, D., Röck, R., Salvenmoser, W., Meyer, D., Bouvier, M., Geley, S., Stefan, E., Aanstad, P. (2021) Feedback Control of the Gpr161-Gαs-PKA axis contributes to basal Hedgehog repression in zebrafish. Development (Cambridge, England). 148(4):
- Yang, L., Webb, S.E., Jin, N., Lee, H.M., Chan, T.F., Xu, G., Chan, J.C.N., Miller, A.L., Ma, R.C.W. (2021) Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish. Journal of diabetes investigation. 12(5):710-727
- Mi, P., Gao, Q., Feng, Z.Y., Zhang, J.W., Zhao, X., Chen, D.Y., Feng, X.Z. (2020) Melatonin attenuates 17β-trenbolone induced insomnia-like phenotype and movement deficiency in zebrafish. Chemosphere. 253:126762
- Arribat, Y., Mysiak, K.S., Lescouzères, L., Boizot, A., Ruiz, M., Rossel, M., Bomont, P. (2019) Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy. The Journal of Clinical Investigation. 129(12):5312-5326
- Ma, P., Song, N.N., Cheng, X., Zhu, L., Zhang, Q., Zhang, L., Yang, X., Wang, H., Kong, Q., Shi, D., Ding, Y.Q., Mao, B. (2019) ZC4H2 stabilizes RNF220 to pattern ventral spinal cord through modulating Shh/Gli signaling. Journal of molecular cell biology. 12(5):337-344
- Yildiz, O., Downes, G.B., Sagerström, C.G. (2019) Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain. Neural Development. 14:5
- Andrzejczuk, L.A., Banerjee, S., England, S.J., Voufo, C., Kamara, K., Lewis, K.E. (2018) Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons. Frontiers in neuroscience. 12:170
1 - 10 of 56
Show