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Fig. 1

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ZDB-FIG-121026-25

Publication

Tanaka et al., 2012 - Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish

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Fig. 1

Loss of function of dpysl2 and dpysl3 inhibited the proper positioning of Rohon-Beard neurons in the dorsal part of the spinal cord. (A–H) Gene expression pattern of dpysl2 (A–D) and dpsyl3 (E–H) at 12 hpf (A, B, E, F) and at 28 hpf (C, D, G, H). Lateral view, anterior left (A, C, E, G) and dorsal view, anterior top (B, D, F, H). Arrowheads in C, D, G, H indicate RB neurons. (I–L) The position of RB neurons in the dorsal spinal cord in the wild-type (I) and the morphants (J–L) at 28 hpf. Dorsal view, anterior top. In the morphants, the position of RB neurons shifted medially compare to the wild-type embryos. (M, N) Quantitative data for the percentage of RB neurons on the midline (M), and total nomber of RB neurons within 3 segments (N). Data are mean ± SEM; ^NNP<0.01, ^NNNP<0.001, different from the wild-type embryos using 2-tailed paired Student′s t-test.

Expression Data

Genes:	dpysl2b dpysl3
Fish:	WT
Anatomical Terms:	neural plate anatomical margin neural plate anterior region neural plate medial region Rohon-Beard neuron spinal cord
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dpysl3 WT + MO3-dpysl2b WT + MO1-dpysl3 + MO3-dpysl2b
Knockdown Reagents:	MO1-dpysl3 MO3-dpysl2b
Observed In:	Rohon-Beard neuron spinal cord axis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 370(2), Tanaka, H., Morimura, R., and Ohshima, T., Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish, 223-236, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.