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ZFIN ID: ZDB-FIG-120109-1
Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms   4(6):763-76 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Protruding-mouth

Fig. 7 Expression of gene transcripts encoding COPII components is reduced in feelgood. (A) Schematic of zebrafish Creb3l2 expression constructs. ‘X’ in the basic domain marks the feelgood N301K mutation. (B) Luciferase reporter assays. Human fibroblasts were transfected with the indicated expression constructs and the firefly luciferase reporter containing 0.8 kb of the zebrafish sec23a promoter. Renilla luciferase control plasmid was also used as transfection efficiency control. Luciferase activities were compared with those of cells transfected with empty vector to determine fold induction. Red line indicates baseline activity adjusted to Renilla luciferase values. (C,D) qPCR analysis of the fold expression change of COPII-related transcripts creb3l2, sec23a, sec23b, sec24d and sec24c (C), and ER-stress-related transcripts s1p, bip and sil1 (D) in feelgood, crusher (sec23a) and bulldog (sec24d) mutants compared with wild types. RNA was extracted form whole embryos at 80 hpf. All results are normalized to β-actin and then to wild type. *P<0.05; **P<0.005.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
creb3l2 creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
hspa5 creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
mbtps1 creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23a creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23b creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24c creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24d creb3l2m662/m662(AB) standard conditions Protruding-mouth whole organism RTPCR
sec23am299/m299(AB) standard conditions Protruding-mouth whole organism RTPCR
sec24dm606/m606(AB) standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.