ZFIN ID: ZDB-FIG-111215-26
Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms   4(6):763-76 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Collagen trafficking is preferentially disrupted in feelgood mutants, leading to notochord defects. (A–F) Immunofluorescence of WGA, Col2α1 and merged images of 15-µm sagittal sections of the notochord of 28-hpf embryos. Arrow indicates vesicle-like collagen staining outside the notochord sheath. (G,H) DIC images of the corresponding sections in A–F. (I–N) Immunofluorescence of type-IV and type-II collagen (I–L) and laminin (M,N) in 15-μm sagittal sections of the notochord at 28 hpf. Scale bar: 5 μm. (O–R) Toluidine blue staining of transverse sections through the notochord at the level of the posterior parachordal plate (O,P) and posterior medulla oblongata (Q,R) in 80-hpf embryos. Schematic diagrams mark the plane of the corresponding sections. Arrows indicate the less robust notochord sheath in feelgood compared with wild types. (S) Immunoblot analysis of type II collagen processing. Molecular forms are indicated as: processed, the fully processed form; pN, pN-collagen II; pNpC, unprocessed procollagen II. (T) EndoH sensitivity assay for N-cadherin. Embryo lysates were either untreated (–) or treated with either EndoH (H) or PNGase F (F) before immunoblotting for N-cadherin.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
creb3l2m662/m662(AB) standard conditions Prim-5 notochord collagen trimer mislocalised, abnormal
Protruding-mouth collagen biosynthetic process decreased process quality, abnormal
Protruding-mouth notochord collagen trimer mislocalised, abnormal
Protruding-mouth notochord extracellular matrix decreased thickness, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.