ZFIN ID: ZDB-FIG-111215-30
Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms   4(6):763-76 Full text @ Dis. Model. Mech.
Anatomical Terms:
Stage Range: 4-cell to Day 5

Fig. S2 Expression of creb3l2 during zebrafish development. (A) RT-PCR analysis of creb3l2 expression with beta-actin as a loading control. (B-E′) Whole mount in situ hybridization of creb3l2 expression. Arrows indicate increased expression at the developing pharyngeal skeleton and fin between 36 and 60 hpf. C-E, lateral views, D′,E′ ventral views of D,E. Abbreviations: e, ethmoid plate; m, Meckel′s cartilage; ch, ceratohyal; oc, otic capsule.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
creb3l2 WT standard conditions 4-cell whole organism RTPCR
64-cell whole organism RTPCR
Shield whole organism ISH
Shield whole organism RTPCR
90%-epiboly whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-25 auditory capsule ISH
Prim-25 pectoral fin ISH
Prim-25 pharyngeal arch cartilage ISH
Prim-25 whole organism RTPCR
Long-pec auditory capsule ISH
Long-pec ceratohyal cartilage ISH
Long-pec ethmoid cartilage ISH
Long-pec Meckel's cartilage ISH
Long-pec pectoral fin ISH
Long-pec whole organism RTPCR
Pec-fin auditory capsule ISH
Pec-fin ceratohyal cartilage ISH
Pec-fin ethmoid cartilage ISH
Pec-fin Meckel's cartilage ISH
Pec-fin pectoral fin ISH
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.