FIGURE

Fig. 6

ID

ZDB-FIG-111215-29

Publication

Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis

Other Figures

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Fig. 6

Decreased Creb3l2 function is responsible for the feelgood phenotype. (A) Schematic representation of the creb3l2 gene structure and positions of the two morpholinos MO-1 and MO-2 used in knockdown experiments. The names, position and orientation of specific primers used to evaluate the effectiveness of MO-2 are shown on top of the genomic locus diagram. Exonic primers are indicated by an ‘e’, intronic primers are indicated by an ‘i’. The predicted truncated form of the protein caused by MO-2 compared with the full-length wild-type protein is shown below (Morphant). PCR amplification products representing spliced and unspliced creb3l2 mRNA as compared with total creb3l2 mRNA in embryos injected with 3 ng MO-2 is shown on the right gel electrophoresis images. The primers used for amplification are shown on the right images. (B–E′) Live (lateral; B–E) and Alcian blue stained (ventral; B′–E′) images of 80-hpf embryos injected with 0.5 ng MO-2 (C,D), or MO-2 plus creb3l2-mRNA (E). NIC, non-injected control is shown in B. (F) Quantification of knockdown and mRNA rescue experiments indicates the percentage of embryos in the four phenotypic classes observed.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-creb3l2
Knockdown Reagent:	MO2-creb3l2
Observed In:	ceratohyal cartilage Meckel's cartilage palatoquadrate cartilage
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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