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ZFIN ID: ZDB-FIG-111215-29
Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms   4(6):763-76 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6 Decreased Creb3l2 function is responsible for the feelgood phenotype. (A) Schematic representation of the creb3l2 gene structure and positions of the two morpholinos MO-1 and MO-2 used in knockdown experiments. The names, position and orientation of specific primers used to evaluate the effectiveness of MO-2 are shown on top of the genomic locus diagram. Exonic primers are indicated by an ‘e’, intronic primers are indicated by an ‘i’. The predicted truncated form of the protein caused by MO-2 compared with the full-length wild-type protein is shown below (Morphant). PCR amplification products representing spliced and unspliced creb3l2 mRNA as compared with total creb3l2 mRNA in embryos injected with 3 ng MO-2 is shown on the right gel electrophoresis images. The primers used for amplification are shown on the right images. (B–E′) Live (lateral; B–E) and Alcian blue stained (ventral; B′–E′) images of 80-hpf embryos injected with 0.5 ng MO-2 (C,D), or MO-2 plus creb3l2-mRNA (E). NIC, non-injected control is shown in B. (F) Quantification of knockdown and mRNA rescue experiments indicates the percentage of embryos in the four phenotypic classes observed.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-creb3l2 standard conditions Protruding-mouth ceratohyal cartilage decreased length, abnormal
Protruding-mouth ceratohyal cartilage malformed, abnormal
Protruding-mouth Meckel's cartilage decreased length, abnormal
Protruding-mouth Meckel's cartilage malformed, abnormal
Protruding-mouth palatoquadrate cartilage decreased length, abnormal
Protruding-mouth palatoquadrate cartilage malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.