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ZFIN ID: ZDB-FIG-111215-24
Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms   4(6):763-76 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1 The feelgood mutation affects craniofacial skeletal development. (A–D) Live images of wild-type (WT) and feelgood (felm662) embryos at 5 dpf. Arrows indicate reduced length of head and trunk in dorsal (A,B) and lateral (C,D) views. (E–F′) Alcian blue staining of cartilage elements in head skeleton in lateral (E,F) and ventral (E′,F′) views. cb, ceratobranchials 3–7; ch, ceratohyal; hs, hyosymplectic; m, Meckel’s cartilage; pq, palatoquadrate. (G,H) Toluidine blue staining of transverse sections of the jaw at the level of the optic nerve (asterisk). Nuclei stain blue, whereas ECM stains purple. Arrow indicates tightly packed nuclei in feelgood mutants.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
creb3l2m662/m662(AB) standard conditions Day 5 ceratohyal cartilage decreased length, abnormal
Day 5 ceratohyal cartilage malformed, abnormal
Day 5 chondroblast spatial pattern, abnormal
Day 5 cranial cartilage extracellular matrix decreased mass, abnormal
Day 5 head decreased length, abnormal
Day 5 Meckel's cartilage decreased length, abnormal
Day 5 Meckel's cartilage malformed, abnormal
Day 5 palatoquadrate cartilage decreased length, abnormal
Day 5 palatoquadrate cartilage malformed, abnormal
Day 5 trunk decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.