ZFIN ID: ZDB-FIG-111215-25
Melville et al., 2011 - The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms   4(6):763-76 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 2 Protein trafficking is disrupted in feelgood mutants. (A–D′′) Immunostaining of WGA (A–D) and Col2α1 (A2–D′) in the Meckel’s cartilage of 80-hpf (A–B′′) and 5-dpf (C–D′′) wild-type (WT; A-A′′, C-C′′) and feelgood. (B-B3, D-D3) embryos. Arrows in merged images indicate aberrant intracellular collagen localization in feelgood. Scale bar: 5 μm. (E–L) TEM images of 80-hpf (E–H) and 5-dpf (I–L) WT and feelgood chondrocytes. Arrows indicate distended ER membranes in feelgood cells. (M–T) TEM images of collagen fibrils in the extracellular space of feelgood mutants and WT siblings at 80 hpf and 4, 5 and 6 dpf. Arrows point to representative individual collagen fibrils. Scale bar: 1 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
creb3l2m662/m662(AB) standard conditions Protruding-mouth chondrocyte endoplasmic reticulum distended, abnormal
Protruding-mouth collagen biosynthetic process decreased process quality, abnormal
Protruding-mouth collagen fibril organization decreased process quality, abnormal
Protruding-mouth cranial cartilage fibrillar collagen trimer decreased mass, abnormal
Protruding-mouth endoplasmic reticulum to Golgi vesicle-mediated transport decreased process quality, abnormal
Protruding-mouth extracellular matrix constituent secretion decreased process quality, abnormal
Protruding-mouth Meckel's cartilage chondrocyte malformed, abnormal
Day 4 collagen fibril organization decreased process quality, abnormal
Day 4 cranial cartilage fibrillar collagen trimer decreased mass, abnormal
Day 5 chondrocyte endoplasmic reticulum distended, abnormal
Day 5 collagen biosynthetic process decreased process quality, abnormal
Day 5 collagen fibril organization decreased process quality, abnormal
Day 5 cranial cartilage fibrillar collagen trimer decreased mass, abnormal
Day 5 endoplasmic reticulum to Golgi vesicle-mediated transport decreased process quality, abnormal
Day 5 extracellular matrix constituent secretion decreased process quality, abnormal
Day 5 Meckel's cartilage chondrocyte malformed, abnormal
Day 6 collagen fibril organization decreased process quality, abnormal
Day 6 cranial cartilage fibrillar collagen trimer decreased mass, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.