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ZIRC
ZFIN ID: ZDB-FIG-110920-84
Keightley et al., 2011 - Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One   6(8):e23845 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Hematopoiesis defects in syr.

Myelomonocytic markers were examined at 23–28 hpf by WISH (A–N); T lymphocyte and thymic epithelium markers were examined at 3.5 dpf in syr and wt (O–R); staining of the thrombocyte marker, cd41 at 3 dpf (S–T). WISH embryos are representative of e4 (median = 21) examples.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
csf1ra AB standard conditions Prim-5 monocyte ISH
med12gl10/gl10 standard conditions Prim-5 monocyte ISH
foxn1 AB standard conditions Protruding-mouth thymus primordium ISH
med12gl10/gl10 standard conditions Protruding-mouth thymus primordium ISH
itga2b AB standard conditions Protruding-mouth thrombocyte ISH
med12gl10/gl10 standard conditions Protruding-mouth Not Detected thrombocyte ISH
lcp1 AB standard conditions Prim-5 myeloid leukocyte ISH
med12gl10/gl10 standard conditions Prim-5 myeloid leukocyte ISH
lyz AB standard conditions Prim-5 myeloid cell ISH
med12gl10/gl10 standard conditions Prim-5 myeloid cell ISH
mmp13a AB standard conditions Prim-5 monocyte ISH
med12gl10/gl10 standard conditions Prim-5 Not Detected monocyte ISH
mpx AB standard conditions Prim-5 neutrophil ISH
med12gl10/gl10 standard conditions Prim-5 Not Detected neutrophil ISH
npsn AB standard conditions Prim-5 granulocyte monocyte progenitor cell ISH
med12gl10/gl10 standard conditions Prim-5 granulocyte monocyte progenitor cell ISH
rag1 AB standard conditions Protruding-mouth T cell ISH
med12gl10/gl10 standard conditions Protruding-mouth Not Detected T cell ISH
spi1b AB standard conditions Prim-5 granulocyte monocyte progenitor cell ISH
med12gl10/gl10 standard conditions Prim-5 granulocyte monocyte progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
med12gl10/gl10 standard conditions Prim-5 neutrophil decreased amount, abnormal
Prim-5 neutrophil differentiation process quality, abnormal
Protruding-mouth T cell absent, abnormal
Protruding-mouth T cell differentiation delayed, abnormal
Protruding-mouth thrombocyte absent, abnormal
Protruding-mouth thrombocyte differentiation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One