FIGURE

Fig. 2

ID

ZDB-FIG-110920-83

Publication

Keightley et al., 2011 - Mediator subunit 12 is required for neutrophil development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Genetic validation of V1046D mutant Med12 underpinning syr phenotype.

(A) Phenocopy of syr by injection of Med12 morpholino; phenotype (PT), genotype (GT) and morpholino (MO) injection are indicated. The upper panel are bright field photos and the lower panels show WT and syr on a Tg(mpx:EGFP) background for enumeration of mpx expressing cells. (B) Rescue of syr neural phenotype with wild-type Med12; phenotype (PT), genotype (GT) and mRNA injected. (C) Rescue of myeloid defect in syr by overexpression of wild-type Med12 mRNA; genotype (GT) and mRNA injected are indicated. (D) Rescue of syr with wild-type (WT) Med12 and Med12 containing the two sequence variations, N1763S and a 12 bp deletion but not the V1046D mutation (SV). Rescue does not occur with injection of V1046D Med12 (MUT) or Med12 containing the two sequence variations in addition to the V1046D mutation (SVM). (E) Non-complementation of syr with trapped (tpd). Rescued mutants were PCR genotype confirmed.

Expression Data

Gene:	GFP
Fish:	i114Tg i114Tg + MO2-med12
Knockdown Reagent:	MO2-med12
Anatomical Term:	neutrophil
Stage:	High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med12^gl10/gl10 AB + MO2-med12 i114Tg + MO2-med12 med12^gl10/+; med12^t25870/+
Knockdown Reagent:	MO2-med12
Observed In:	brain extension eye myeloid leukocyte myeloid leukocyte differentiation ventricular system
Stage:	High-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One