FIGURE

Fig. 2

ID

ZDB-FIG-110428-11

Publication

Bussmann et al., 2011 - Arterial-venous network formation during brain vascularization involves hemodynamic regulation of chemokine signaling

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Fig. 2

VegfA and Notch signaling are required for angiogenic sprouting in the zebrafish hindbrain. (A-D) Whole-mount in situ hybridization showing the expression of vegfaa (A), vegfab (B), kdrl (C) and dll4 (D) at 36 hpf. (E-L) Maximal intensity projections of confocal z-stacks at 36 (E,F,K,L), 54 (I,J) or 60 (G,H) hpf. Arrowheads indicate CtA sprouts emerging from the PHBC. Arrow indicates the position of the BA. (E,F) Sibling (E) or kdrl mutant (F) embryos, with endothelial cells labeled by kdrl:mem-rfp expression. (G,H) kdrl:mem-rfp-expressing embryos injected with control MO (G) or dll4 MO (H). (I,J) kdrl:gfp-expressing embryos treated from 30 to 54 hpf with DMSO (I) or DAPT (J). (K,L) Embryos from a cross between cdh5:gal4ff, uas:gfp and uas:NICD. The embryo in K is negative for uas:NICD, whereas that in L is heterozygous for uas:NICD. Endothelial cells are labeled by cdh5:gal4ff, uas:gfp expression. Dorsal views, anterior to the left.

Expression Data

Genes:	dll4 EGFP kdrl mCherry vegfaa vegfab
Fish:	WT s843Tg s916Tg mu101Tg; nkuasgfp1aTg kca3Tg; mu101Tg; nkuasgfp1aTg s916Tg + MO2-dll4 kdrl^{hu5088/hu5088}; s916Tg
Condition:	chemical treatment: DAPT
Knockdown Reagent:	MO2-dll4
Anatomical Terms:	basilar artery blood vessel endothelium brain vasculature central artery blood vessel endothelium hindbrain hindbrain floor plate primordial hindbrain channel blood vessel endothelium
Stage Range:	Prim-25 to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg kca3Tg; mu101Tg; nkuasgfp1aTg s916Tg + MO2-dll4 kdrl^{hu5088/hu5088}; s916Tg
Condition:	chemical treatment: DAPT
Knockdown Reagent:	MO2-dll4
Observed In:	basilar artery blood vessel endothelial cell central artery sprouting angiogenesis
Stage Range:	Prim-25 to Pec-fin

Phenotype Detail

Acknowledgments

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