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ZIRC
ZFIN ID: ZDB-FIG-110428-15
Bussmann et al., 2011 - Arterial-venous network formation during brain vascularization involves hemodynamic regulation of chemokine signaling. Development (Cambridge, England)   138(9):1717-1726 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6

Flow is required for hindbrain vascular patterning and downregulation of cxcr4a expression. (A,B) Maximal intensity projections of confocal z-stacks of kdrl:mem-rfp transgenic zebrafish embryos at 52 hpf, untreated (A) or treated for 8 hours with 2× tricaine (B). Arrowheads indicate the presence of filopodia on non-perfused CtAs. (C,D) In situ hybridization for cxcr4a mRNA in untreated control embryos fixed at 48 hpf (C) or embryos treated for 1 hour with 2× tricaine from 47-48 hpf and fixed at 48 hpf (D). (E) Quantitative analysis of cxcr4a mRNA expression. cxcr4a-expressing cells were counted in embryos following cxcr4a in situ hybridization. P<0.05 (Mann-Whitney U test). n=15 embryos per group. Error bars represent s.d. (F-H) cxcr4a in situ hybridization in cxcl12bmu100 (F) and cxcr4aum21 (G) homozygous mutant embryos or embryos injected with 2 ng tnnt2 MO at the 1-cell stage (H) and fixed at 48 hpf. (I) Two-color fluorescent in situ hybridization of embryos injected with 2 ng tnnt2 MO and fixed at 48 hpf, showing the distribution cxcr4a mRNA (red) and kdrl mRNA (green). (J) Angiography using Quantum dots (Qdot-655; Invitrogen) in a kdrl:gfp transgenic, cxcl12bmu100 homozygous mutant embryo at 52 hpf. In this embryo, only one half of the brain had perfused CtAs (flow+ versus flow). (K) In situ hybridization for cxcr4a mRNA in a cxcl12bmu100 homozygous mutant embryo in which half of the hindbrain contained perfused CtAs at 48 hpf. Dorsal views, anterior to the left, except in I, which is a side view with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr4a WT control Long-pec central artery blood vessel endothelium ISH
WT chemical treatment: drug Long-pec central artery blood vessel endothelium ISH
WT + MO1-tnnt2a standard conditions Long-pec basilar artery blood vessel endothelium ISH
Long-pec central artery blood vessel endothelium ISH
Long-pec posterior communicating artery blood vessel endothelium ISH
cxcl12bmu100/mu100 standard conditions Long-pec central artery blood vessel endothelium ISH
cxcr4aum21/um21 standard conditions Long-pec central artery blood vessel endothelium ISH
EGFP cxcl12bmu100; s843Tg standard conditions Long-pec central artery blood vessel endothelium IFL
kdrl WT + MO1-tnnt2a standard conditions Long-pec basilar artery blood vessel endothelium ISH
Long-pec central artery blood vessel endothelium ISH
Long-pec posterior communicating artery blood vessel endothelium ISH
Long-pec primordial hindbrain channel blood vessel endothelium ISH
mCherry s916Tg standard conditions Long-pec central artery blood vessel endothelium IFL
Long-pec primordial hindbrain channel blood vessel endothelium IFL
s916Tg chemical treatment: drug Long-pec central artery blood vessel endothelium IFL
Long-pec primordial hindbrain channel blood vessel endothelium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: drug Long-pec blood circulation decreased rate, abnormal
Long-pec central artery physical object quality, abnormal
Long-pec sprouting angiogenesis process quality, abnormal
WT + MO1-tnnt2a standard conditions Long-pec blood circulation arrested, abnormal
Long-pec central artery physical object quality, abnormal
Long-pec sprouting angiogenesis process quality, abnormal
cxcl12bmu100/mu100 standard conditions Long-pec central artery physical object quality, abnormal
Long-pec sprouting angiogenesis process quality, abnormal
cxcl12bmu100; s843Tg standard conditions Long-pec central artery physical object quality, abnormal
Long-pec sprouting angiogenesis process quality, abnormal
cxcr4aum21/um21 standard conditions Long-pec central artery physical object quality, abnormal
Long-pec sprouting angiogenesis process quality, abnormal
s916Tg chemical treatment: drug Long-pec blood circulation decreased rate, abnormal
Long-pec central artery physical object quality, abnormal
Long-pec sprouting angiogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development