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ZFIN ID: ZDB-FIG-110112-28
Clément et al., 2011 - The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development (Cambridge, England)   138(2):291-302 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Long-pec

Fig. 2 Effect of inhibition of Cdc14B function on LR asymmetry. (A-L) In situ hybridization and expression pattern quantification in non-injected and cdc14b MO-injected zebrafish embryos of nkx2.5 (A-D), foxa3 (E-H) and spaw (I-L) at 31 hpf, 50 hpf and 19 hpf, respectively. (M) Quantification of LR asymmetry defects in non-injected and 0.5 ng or 1.5 ng MO1-cdc14b-injected wild-type and cdc14bvu426 homozygous mutant embryos at 31 hpf using cmlc2:GFP transgene. (N) Rescue of the LR asymmetry phenotype in MO1-cdc14b-injected embryos. LR asymmetry defects were quantified using spaw expression pattern at 18 hpf. Numbers of embryos analyzed are indicated (n). Data are mean ± s.e.m. Statistics were carried out using Student′s t-test (D,H,L,M) and paired Student′s t-test (N), and are only presented for the left-sided heart class in M and the spaw left expression class in N.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa3 WT standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
WT + MO1-cdc14b standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
WT + MO2-cdc14b standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
nkx2.5 WT standard conditions Prim-15 heart tube ISH
WT + MO1-cdc14b standard conditions Prim-15 heart tube ISH
WT + MO2-cdc14b standard conditions Prim-15 heart tube ISH
spaw WT standard conditions 20-25 somites lateral mesoderm ISH
WT + MO1-cdc14b standard conditions 20-25 somites Not Detected lateral mesoderm ISH
WT + MO2-cdc14b standard conditions 20-25 somites mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdc14b standard conditions 20-25 somites embryonic heart tube left/right pattern formation disrupted, abnormal
WT + MO1-cdc14b standard conditions Prim-15 left/right pattern formation occurrence, abnormal
WT + MO1-cdc14b standard conditions Long-pec heart looping disrupted, abnormal
WT + MO2-cdc14b standard conditions 20-25 somites embryonic heart tube left/right pattern formation disrupted, abnormal
Prim-15 left/right pattern formation occurrence, abnormal
Long-pec heart looping disrupted, abnormal
cdc14bvu426Tg/vu426Tg; twu34Tg standard conditions Prim-15 left/right pattern formation occurrence, normal
cdc14bvu426Tg/vu426Tg; twu34Tg + MO1-cdc14b standard conditions Prim-15 embryonic heart tube left/right pattern formation disrupted, abnormal
twu34Tg + MO1-cdc14b standard conditions Prim-15 left/right pattern formation occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development