ZFIN ID: ZDB-FIG-110112-33
Clément et al., 2011 - The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development (Cambridge, England)   138(2):291-302 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 75%-epiboly to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 10-13 somites

Fig. 7 Interaction between the FGF pathway and Cdc14B. Expression pattern of erm1 (A,B) and pea3 (C,D) at 8 hpf, and pax2a (E,F) at 17 hpf. (G-J) KV cilia in siblings and fgf8/acex15 homozygous mutant embryos, non-injected (G,H) or injected with MO1-cdc14b (I,J) at 14 hpf visualized with an anti-Ac-tubulin antibody. (K) Cilia length was determined at 14 hpf. The numbers of cilia analyzed are indicated. Data are mean ± s.e.m. Statistics were carried out using Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 WT standard conditions 75%-epiboly forerunner cell group ISH
WT + MO1-cdc14b standard conditions 75%-epiboly forerunner cell group ISH
etv5b WT standard conditions 75%-epiboly forerunner cell group ISH
WT + MO1-cdc14b standard conditions 75%-epiboly forerunner cell group ISH
pax2a WT standard conditions 14-19 somites immature eye ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
14-19 somites pronephros ISH
WT + MO1-cdc14b standard conditions 14-19 somites immature eye ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
14-19 somites pronephros ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdc14b standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
fgf8ax15/+ standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
fgf8ax15/+ + MO1-cdc14b standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
fgf8ax15/x15 standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
fgf8ax15/x15 + MO1-cdc14b standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development