FIGURE

Fig. 3

ID

ZDB-FIG-110112-29

Publication

Clément et al., 2011 - The Cdc14B phosphatase contributes to ciliogenesis in zebrafish

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Fig. 3

Cdc14B loss- and gain-of-function effect on the KV. (A,B) KV at 10 hpf visualized using Sox17-GFP. (C) The number of cells in the KV was determined in non-injected and cdc14b MO-injected zebrafish embryos at the indicated times. (D,E) Cilia in the KV at 14 hpf visualized with an anti-acetylated-tubulin (Ac-tubulin) antibody. (F,G) Cilia numbers (F) and cilia length (G) were determined at 14 hpf. The numbers of embryos (C,F) and cilia (G) analyzed are indicated. Data are mean ± s.e.m. Statistics were carried out using Student′s t-test. Unless otherwise shown, statistics were calculated against the NI sample. (H,I) Particle paths in the KV of a single non-injected (H) or MO1-cdc14b-injected (I) embryo at 14 hpf. Three embryos were analyzed per condition.

Expression Data

Gene:	GFP
Fish:	s870Tg s870Tg + MO1-cdc14b
Knockdown Reagent:	MO1-cdc14b
Anatomical Term:	forerunner cell group
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s870Tg + MO1-cdc14b s870Tg + MO2-cdc14b s870Tg + MO2-cdc14b + MO3-cdc14b s870Tg + MO3-cdc14b
Knockdown Reagents:	MO1-cdc14b MO2-cdc14b MO3-cdc14b
Observed In:	forerunner cell group Kupffer's vesicle cilium
Stage Range:	Bud to 10-13 somites

Phenotype Detail

Acknowledgments

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