ZFIN ID: ZDB-FIG-100427-27
Binot et al., 2010 - Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells. Developmental Biology   340(2):397-407 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 3 nkx6.1 and nkx6.2 pancreatic expression at 24 hpf. A–P: Double fluorescent WISHs showing expression at 24 hpf of nkx6.1 and glucagon (gcg) (A), insulin (ins) (B), somatostatin (sst) (C), ghrelin (ghr) (D), neuroD (E), sox4b (F) or pdx1 (G), of nkx6.2 and glucagon (H), insulin (I), somatostatin (J), ghrelin (K), neuroD (L), sox4b (M) or isl1 (N) and of isl1 and sox4b (O) or all the pancreatic hormone genes (P). Z-plane confocal images. Scale: 20 μm. Q: Schematic representation of pancreatic organization at 24 hpf. All views are lateral with anterior part to the left. h: hypochord.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga AB standard conditions Prim-5 posterior pancreatic bud ISH
ghrl AB standard conditions Prim-5 posterior pancreatic bud ISH
ins AB standard conditions Prim-5 posterior pancreatic bud ISH
isl1a AB standard conditions Prim-5 posterior pancreatic bud ISH
neurod1 AB standard conditions Prim-5 pancreatic bud ISH
nkx6.1 AB standard conditions Prim-5 posterior pancreatic bud ISH
nkx6.2 AB standard conditions Prim-5 pancreatic bud ventral side ISH
pdx1 AB standard conditions Prim-5 gut ISH
Prim-5 pancreatic bud ISH
sox4b AB standard conditions Prim-5 pancreatic bud ISH
sst2 AB standard conditions Prim-5 posterior pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.