ZFIN ID: ZDB-FIG-100427-28
Binot et al., 2010 - Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells. Developmental Biology   340(2):397-407 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 4 Effects of nkx6 knockdown on endocrine cells. A–P: Ventral views of WISHs showing expression of glucagon at 30 hpf (A, E, I and M), insulin at 24 hpf (B, F, J and N), somatostatin at 24 hpf (C, G, K and O), and ghrelin at 30 hpf (D, H, L and P) in control (A–D), nkx6.1 (E–H), nkx6.2 (I–L), and nkx6.1/nkx6.2 (M–P) morphants. Scale: 20 μm. Q–S: Number of cells expressing glucagon at 30 hpf in nkx6.1 (Q) and nkx6.2 (R) morphants (the horizontal line represents the mean) and number of cells expressing the different pancreatic hormones at 30 hpf in nkx6.1/nkx6.2 morphants (S). The presented data are representative of at least three reproducible and independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gcga AB control Prim-15 glucagon secreting cell ISH
AB + MO1-nkx6.1 + MO2-nkx6.1 standard conditions Prim-15 glucagon secreting cell ISH
AB + MO2-nkx6.1 + MO3-nkx6.2 standard conditions Prim-15 Not Detected glucagon secreting cell ISH
AB + MO3-nkx6.2 + MO4-nkx6.2 standard conditions Prim-15 glucagon secreting cell ISH
ghrl AB control Prim-15 ghrelin secreting cell ISH
AB + MO1-nkx6.1 + MO2-nkx6.1 standard conditions Prim-15 ghrelin secreting cell ISH
AB + MO2-nkx6.1 + MO3-nkx6.2 standard conditions Prim-15 ghrelin secreting cell ISH
AB + MO3-nkx6.2 + MO4-nkx6.2 standard conditions Prim-15 ghrelin secreting cell ISH
ins AB control Prim-5 insulin secreting cell ISH
AB + MO1-nkx6.1 + MO2-nkx6.1 standard conditions Prim-5 insulin secreting cell ISH
AB + MO2-nkx6.1 + MO3-nkx6.2 standard conditions Prim-5 insulin secreting cell ISH
AB + MO3-nkx6.2 + MO4-nkx6.2 standard conditions Prim-5 insulin secreting cell ISH
sst2 AB control Prim-5 somatostatin secreting cell ISH
AB + MO1-nkx6.1 + MO2-nkx6.1 standard conditions Prim-5 somatostatin secreting cell ISH
AB + MO2-nkx6.1 + MO3-nkx6.2 standard conditions Prim-5 somatostatin secreting cell ISH
AB + MO3-nkx6.2 + MO4-nkx6.2 standard conditions Prim-5 somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-nkx6.1 + MO2-nkx6.1 standard conditions Prim-15 pancreatic bud has fewer parts of type pancreatic A cell, abnormal
Prim-15 pancreatic bud has normal numbers of parts of type pancreatic B cell, normal
AB + MO2-nkx6.1 + MO3-nkx6.2 standard conditions Prim-15 pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Prim-15 pancreatic bud lacks parts or has fewer parts of type pancreatic A cell, abnormal
AB + MO3-nkx6.2 + MO4-nkx6.2 standard conditions Prim-15 pancreatic bud has fewer parts of type pancreatic A cell, abnormal
Prim-15 pancreatic bud has normal numbers of parts of type pancreatic B cell, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.