FIGURE

Fig. 4

ID

ZDB-FIG-100427-28

Publication

Binot et al., 2010 - Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells

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Fig. 4

Effects of nkx6 knockdown on endocrine cells. A–P: Ventral views of WISHs showing expression of glucagon at 30 hpf (A, E, I and M), insulin at 24 hpf (B, F, J and N), somatostatin at 24 hpf (C, G, K and O), and ghrelin at 30 hpf (D, H, L and P) in control (A–D), nkx6.1 (E–H), nkx6.2 (I–L), and nkx6.1/nkx6.2 (M–P) morphants. Scale: 20 μm. Q–S: Number of cells expressing glucagon at 30 hpf in nkx6.1 (Q) and nkx6.2 (R) morphants (the horizontal line represents the mean) and number of cells expressing the different pancreatic hormones at 30 hpf in nkx6.1/nkx6.2 morphants (S). The presented data are representative of at least three reproducible and independent experiments.

Expression Data

Genes:	gcga ghrl ins sst2
Fish:	AB AB + MO1-nkx6.1 + MO2-nkx6.1 AB + MO3-nkx6.2 + MO4-nkx6.2 AB + MO2-nkx6.1 + MO3-nkx6.2
Knockdown Reagents:	MO1-nkx6.1 MO2-nkx6.1 MO3-nkx6.2 MO4-nkx6.2
Anatomical Terms:	ghrelin secreting cell glucagon secreting cell insulin secreting cell somatostatin secreting cell
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-nkx6.1 + MO2-nkx6.1 AB + MO3-nkx6.2 + MO4-nkx6.2 AB + MO2-nkx6.1 + MO3-nkx6.2
Knockdown Reagents:	MO1-nkx6.1 MO2-nkx6.1 MO3-nkx6.2 MO4-nkx6.2
Observed In:	pancreatic A cell pancreatic B cell pancreatic bud
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.