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TERT is required for both primitive and definitive hematopoiesis in the zebrafish. Whole-mount in situ hybridization of control and TERT deficient zebrafish embryos at the 20-somite stage (19 hpf) (A), and at 28–32 hpf (B). (A) For the detection of primitive hematopoietic markers, 20-somite stage zebrafish embryos (19 hpf) are shown and are oriented anterior left in lateral (a–f) and dorsal views (a′–d′). Control and TERT morphants were analyzed for the expression of scl, lmo2 and gata-2, which are early hematopoietic markers. The expression of scl and lmo2 in the ICM (a–d, a′–d′), and that of gata-2 in the blood island (e, f), is indicated by arrowheads. (B) Embryos at 28–32 hpf and oriented anterior left in a lateral view and are shown at a low (a–r) and high (a′–r′) magnification of the trunk region. Control and TERT morphants were analyzed for the expression of multiple hematopoietic cell lineage markers. Representative time points for the expression of runx1 (32 hpf), c-myb (32 hpf), alas2 (32 hpf), gata-1 (28 hpf), pu.1 (28 hpf), globin bE3 (28 hpf), l-plastin (28 hpf), mpo (28 hpf), and flk1 (28 hpf) genes are shown. The expression in the arterial region is indicated by arrowheads (a′–n′).
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