FIGURE

Fig. S5

ID

ZDB-FIG-081028-40

Publication

Imamura et al., 2008 - A non-canonical function of zebrafish telomerase reverse transcriptase is required for developmental hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Analyses of Telomere length in zTERT morphants.
(A) Metaphase spreads from zebrafish embryos for quantitative telomere FISH (Q-FISH) analysis. Metaphase chromosomes from Cont-MO1-injected embryos (a), and zTERT-MO1-injected embryos (b), for telomere Q-FISH.
(B) Representative histograms showing the distribution of telomere lengths by telomere fluorescence intensity measurements of metaphase spreads from control and zTERT morphants (MO1 and MO2; 8 ng) at 24 hpf, as measured using the TFL-TELO software. Histograms of telomere Q-FISH for Cont-MO1 and zTERT-MO1 (a), and Cont-MO2 and zTERT-MO2 (b). The x-axis depicts the intensity of each signal as expressed in telomere fluorescence intensity units (TFU), and the y-axis shows the frequency of telomeres of a given intensity. The dashed orange line indicates 10 x 10² TFU.
(C) Telomeric restriction fragment (TRF) analysis of DNA isolated from either uninjected, Cont-MO1-, or zTERT-MO1-injected embryos (a) , and from zebrafish genomic DNA digested by DNase I in vitro and control human DNA (DNA-high and DNA-low) (b) .

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One