Two-dimensional electrophoresis (2-DE) profiles of total adult zebrafish lens protein. Separation was performed on 11 cm IPG strips with pH gradients of 5–8 (A), 3–10 nonlinear (B) and 7–10 (C). Gels were Coomassie stained and spots were identified by MALDI-TOF MS. Mass standards are indicated on the left in kiloDaltons. Spots labeled with a “?” contain two or more crystallins from the indicated family. Accession numbers for each crystallin are shown in Table 1. The accession numbers for four identified non-crystallins are as follows: actin (AAO38846), BTB (POZ) domain containing protein 2 (NP_001038557), proliferation associated nuclear panel 1 (Pane1; NP_991153), and galectin-related inter-fiber protein (XP_684452).