ZFIN ID: ZDB-FIG-050323-14
Bernardos et al., 2005 - Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina. Developmental Biology   278(2):381-395 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Pec-fin

Fig. 3 Müller glia differentiation is inhibited in mib-/- retinas at 65 hpf. Müller glia in the retina (outlined) expressed zrf-1/GFAP (arrowhead) as did astrocytes and/or radial glia in the brain (*) of wild-type embryos (A). In contrast, zrf-1/GFAP expression was absent in the retina (outlined) but present in the brain (*) of mib-/- embryos (B; N = 10). (C, D) Expression of her6/hes1 in wild-type and mib-/- 65 retinas. (C′, D′) Differential interference contrast (DIC) images of the retinas in (C and D; N = 8 embryos). (C, C′) In the wild-type embryos, her6/hes1 expression was present in the brain (*) and in the germinal zone (arrowhead), lens epithelium (small arrow) and inner nuclear layer (large arrow) of the retina. (D, D′) The mib-/- embryos expressed her6/hes1 in the brain (*) but expression in the eye was restricted to the lens epithelium (small arrow). Scale bar = 50 μm (A–D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gfap WT standard conditions Pec-fin glial cell IHC
Pec-fin retina IHC
mib1ta52b/ta52b standard conditions Pec-fin glial cell IHC
Pec-fin Not Detected retina IHC
her6 WT standard conditions Pec-fin brain ISH
Pec-fin lens ISH
Pec-fin retina ISH
mib1ta52b/ta52b standard conditions Pec-fin brain ISH
Pec-fin lens ISH
Pec-fin Not Detected retina ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 278(2), Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A., Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina, 381-395, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.