ZFIN Figure: Bernardos et al., 2005, Fig. 3

ZFIN ID: ZDB-FIG-050323-14

ZFIN ID: ZDB-FIG-050323-14

Bernardos et al., 2005 - Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina. Developmental Biology 278(2):381-395 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	gfap her6
Fish:	WT mib1^ta52b/ta52b
Anatomical Terms:	brain glial cell lens retina
Stage:	Pec-fin

Fig. 3 Müller glia differentiation is inhibited in mib^-/- retinas at 65 hpf. Müller glia in the retina (outlined) expressed zrf-1/GFAP (arrowhead) as did astrocytes and/or radial glia in the brain (*) of wild-type embryos (A). In contrast, zrf-1/GFAP expression was absent in the retina (outlined) but present in the brain (*) of mib^-/- embryos (B; N = 10). (C, D) Expression of her6/hes1 in wild-type and mib^-/- 65 retinas. (C′, D′) Differential interference contrast (DIC) images of the retinas in (C and D; N = 8 embryos). (C, C′) In the wild-type embryos, her6/hes1 expression was present in the brain (*) and in the germinal zone (arrowhead), lens epithelium (small arrow) and inner nuclear layer (large arrow) of the retina. (D, D′) The mib^-/- embryos expressed her6/hes1 in the brain (*) but expression in the eye was restricted to the lens epithelium (small arrow). Scale bar = 50 μm (A–D).

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
gfap	WT	standard conditions	Pec-fin		glial cell	IHC
			Pec-fin		retina	IHC
	mib1^ta52b/ta52b	standard conditions	Pec-fin		glial cell	IHC
			Pec-fin	Not Detected	retina	IHC
her6	WT	standard conditions	Pec-fin		brain	ISH
			Pec-fin		lens	ISH
			Pec-fin		retina	ISH
	mib1^ta52b/ta52b	standard conditions	Pec-fin		brain	ISH
			Pec-fin		lens	ISH
			Pec-fin	Not Detected	retina	ISH

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 278(2), Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A., Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina, 381-395, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 278(2), Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A., Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina, 381-395, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.