ZFIN Figure: Bernardos et al., 2005, Fig. 1

ZFIN ID: ZDB-FIG-050323-12

ZFIN ID: ZDB-FIG-050323-12

Bernardos et al., 2005 - Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina. Developmental Biology 278(2):381-395 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	alcama rho
Fish:	WT mib1^ta52b/ta52b
Anatomical Terms:	cranial nerve II retina retinal ganglion cell layer retinal photoreceptor layer
Stage:	Pec-fin

Fig. 1 Differentiation and laminar organization of the retina in wild-type and mib^-/- embryos at 65 hpf. DAPI-stained wild-type (A) and mib^-/- (B) retinas. Lamination was absent in the mib^-/- retina except for an outer nuclear layer (onl; arrow). TUNEL-labeled wild-type (C) and mib^-/- (D) retinas (Fast Red—red). Representative apoptotic cells are indicated by arrows in the wild-type. Ganglion cells labeled with the zn-5/neurolin antibody in wild-type (E) and mib^-/- (F) retinas (Cy-3—red; arrowhead = optic nerve; N = 10 embryos examined for each condition). (G–J) Opsin expression in wild-type and mib^-/- retinas. Expression of red opsin (Cy-3—red) in wild-type (G) and mib^-/- (H) retinas (N = 10 embryos). Expression of rod opsin (FITC—green) in rod photoreceptors in wild-type (I) and mib^-/- (J) retinas (N = 10 embryos). Abbreviations: ganglion cell layer (gcl); inner nuclear layer (inl); outer nuclear layer (onl). Dorsal is up and ventral is down in this and all subsequent figures unless otherwise noted. The lens and outer border of the retina in this figure and all subsequent figures has been outlined to orient the reader. Scale bar = 50 μm (A–J).

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
alcama	WT	standard conditions	Pec-fin	cranial nerve II	IHC
			Pec-fin	retinal ganglion cell layer	IHC
	mib1^ta52b/ta52b	standard conditions	Pec-fin	cranial nerve II	IHC
			Pec-fin	retina	IHC
rho	WT	standard conditions	Pec-fin	retinal photoreceptor layer	IHC
	mib1^ta52b/ta52b	standard conditions	Pec-fin	retina	IHC

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 278(2), Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A., Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina, 381-395, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 278(2), Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A., Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina, 381-395, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.