ZFIN ID: ZDB-FIG-050323-12
Bernardos et al., 2005 - Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina. Developmental Biology   278(2):381-395 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Pec-fin

Fig. 1 Differentiation and laminar organization of the retina in wild-type and mib-/- embryos at 65 hpf. DAPI-stained wild-type (A) and mib-/- (B) retinas. Lamination was absent in the mib-/- retina except for an outer nuclear layer (onl; arrow). TUNEL-labeled wild-type (C) and mib-/- (D) retinas (Fast Red—red). Representative apoptotic cells are indicated by arrows in the wild-type. Ganglion cells labeled with the zn-5/neurolin antibody in wild-type (E) and mib-/- (F) retinas (Cy-3—red; arrowhead = optic nerve; N = 10 embryos examined for each condition). (G–J) Opsin expression in wild-type and mib-/- retinas. Expression of red opsin (Cy-3—red) in wild-type (G) and mib-/- (H) retinas (N = 10 embryos). Expression of rod opsin (FITC—green) in rod photoreceptors in wild-type (I) and mib-/- (J) retinas (N = 10 embryos). Abbreviations: ganglion cell layer (gcl); inner nuclear layer (inl); outer nuclear layer (onl). Dorsal is up and ventral is down in this and all subsequent figures unless otherwise noted. The lens and outer border of the retina in this figure and all subsequent figures has been outlined to orient the reader. Scale bar = 50 μm (A–J).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama WT standard conditions Pec-fin cranial nerve II IHC
Pec-fin retinal ganglion cell layer IHC
mib1ta52b/ta52b standard conditions Pec-fin cranial nerve II IHC
Pec-fin retina IHC
rho WT standard conditions Pec-fin retinal photoreceptor layer IHC
mib1ta52b/ta52b standard conditions Pec-fin retina IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 278(2), Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A., Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina, 381-395, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.