PUBLICATION

Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina

Authors
Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A.
ID
ZDB-PUB-050202-2
Date
2005
Source
Developmental Biology   278(2): 381-395 (Journal)
Registered Authors
Bernardos, Rebecca, Lentz, Steve, Raymond, Pamela
Keywords
Notch; Delta; mind bomb; Retinal development; Photoreceptors; Müller glia; ?-secretase inhibitors her; Hes
MeSH Terms
  • Animals
  • Body Patterning/physiology*
  • Cell Death
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/physiology
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins/physiology*
  • Microscopy, Fluorescence
  • Mutation
  • Neuroglia/physiology*
  • Receptors, Notch
  • Retina/cytology
  • Retina/embryology*
  • Retinal Cone Photoreceptor Cells/embryology
  • Retinal Rod Photoreceptor Cells/embryology
  • Signal Transduction/physiology
  • Ubiquitin-Protein Ligases/deficiency
  • Ubiquitin-Protein Ligases/genetics
  • Zebrafish/embryology
  • Zebrafish Proteins/physiology
PubMed
15680358 Full text @ Dev. Biol.
Abstract
Notch-Delta signaling has been implicated in several alternative modes of function in the vertebrate retina. To further investigate these functions, we examined retinas from zebrafish embryos in which bidirectional Notch-Delta signaling was inactivated either by the mind bomb (mib) mutation, which disrupts E3 ubiquitin ligase activity, or by treatment with gamma-secretase inhibitors, which prevent intramembrane proteolysis of Notch and Delta. We found that inactivating Notch-Delta signaling did not prevent differentiation of retinal neurons, but it did disrupt spatial patterning in both the apical-basal and planar dimensions of the retinal epithelium. Retinal neurons differentiated, but their laminar arrangement was disrupted. Photoreceptor differentiation was initiated normally, but its progression was slowed. Although confined to the apical retinal surface as in normal retinas, the planar organization of cone photoreceptors was disrupted: cones of the same spectral subtype were clumped rather than regularly spaced. In contrast to neurons, Muller glia failed to differentiate suggesting an instructive role for Notch-Delta signaling in gliogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes