Enhancer Trap Construct

Et(hsp70l:GAL4FF)

ID

ZDB-ETCONSTRCT-080529-1

Name

Et(hsp70l:GAL4FF)

Previous Names

T2KhspGFF (1)
Et(hsp70l:Gal4FF)

Type

engineered_plasmid

Regulatory Regions

hsp70l (1)

Coding Sequences

GAL4FF (1)

Contains Sequences

T2K (1)
UAS (1)

Note

Enhancer trap construct incorporating medaka Tol2 transposable element. A heat-shock promoter drives expression of the DNA-binding domain from Gal4 and two transcription activating modules from VP16.

Plasmid Map

Genomic Features

That Utilize Et(hsp70l:GAL4FF) Construct

Genomic Feature	Affected Genomic Regions
js101Tg
nkhsp70GFF4AEt
nkhspGFF15ATg
nkhspGFF28bEt
nkhspGFF3AEt
nkhspGFF53AEt
nkhspGFF55BEt
nkhspGFF57AEt
nkhspGFF62ATg

Transgenics

That Utilize Et(hsp70l:GAL4FF) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
bc3Tg; nkhspGFF53AEt; nkuasgfp1aTg			Fig. 5 from Pujol-Martí et al., 2012
c264Tg; nkhsp70GFF4AEt; vo8Tg		Fig. 3 from Dow et al., 2015	Fig. 3 from Dow et al., 2015
celsr3^fh339/fh339; nkhspGFF62ATg; p201Tg	celsr3	Fig. 2 from Bremer et al., 2019
chi4Tg; nkhspGFF15ATg			Fig. 6 from Mizoguchi et al., 2017
cntnap2a^nkhgn39dEt; nkhspGFF53AEt	cntnap2a
cyfip2^p400/p400; mycbp2^{tn207b/tn207b}; nkhspGFF62ATg; p201Tg	cyfip2 mycbp2	Fig. 8 from Bremer et al., 2019
cyfip2^p400/p400; nkhspGFF62ATg; p201Tg	cyfip2	Fig. 2 from Bremer et al., 2019
dync1h1^{hi3684Tg/hi3684Tg}; nkhspGFF62ATg; p201Tg	dync1h1	Fig. 2 from Bremer et al., 2019
gja2^mi264/mi264; nkGCaMPHS4aTg; nkhspGFF28bEt	gja2	Fig. 7 from Hirata et al., 2012	Fig. 7 from Hirata et al., 2012
js101Tg
kca3Tg; nkhspGFF53AEt; nkuasgfp1aTg		Fig. 6 from Faucherre et al., 2011	Fig. 6 from Faucherre et al., 2011
kcna1a^p181/p181; nkhspGFF62ATg; y362Tg; zf206Et	kcna1a	Fig 5 from Meserve et al., 2021	Fig 5 from Meserve et al., 2021
mib1^ta52b/ta52b; chi4Tg; nkhspGFF15ATg	mib1	Fig. 6 from Mizoguchi et al., 2017	Fig. 6 from Mizoguchi et al., 2017
mitfa^w2/w2; mpv17^a9/a9; nkhspGFF3AEt; zf3136Tg	mitfa mpv17		Fig. 4 from Yamada et al., 2019
mitfa^w2/w2; mpv17^a9/a9; nkhspGFF3AEt; zf3137Tg	mitfa mpv17	Fig. 4 from Yamada et al., 2019	Fig. 4 from Yamada et al., 2019
mycbp2^tn207b/+; mycbp2^tp203/+; nkhspGFF62ATg; p201Tg	mycbp2	Fig. 4 from Bremer et al., 2019
mycbp2^{tn207b/tn207b}; nkhspGFF62ATg; p201Tg	mycbp2	4 figures from Bremer et al., 2019
mycbp2^{tn207b/tn207b}; nkhspGFF62ATg; p411Tg; ue2Tg	mycbp2		Fig. 6 from Bremer et al., 2019
mycbp2^{tn207b/tn207b}; nkhspGFF62ATg; p411Tg; zf167Tg	mycbp2		Fig. 6 from Bremer et al., 2019
mycbp2^{tn207b/tn207b}; nkhspGFF62ATg; p412Tg	mycbp2	Fig. 7 from Bremer et al., 2019
nig6Tg; nkhspGFF62ATg			Fig. S4 from Yamanaka et al., 2013
nig7Tg; nkhspGFF62ATg			Fig. S4 from Yamanaka et al., 2013
nkGCaMPHS4aTg; nkhspGFF28bEt			Fig. 7 from Hirata et al., 2012
nkhsp70GFF4AEt
nkhspGFF28bEt; nkuasrfp1aTg
nkhspGFF3AEt			Fig. 6 from Nagata et al., 2017
nkhspGFF3AEt + MO1-atp5md + MO2-atp5md		4 figures from Nagata et al., 2017	Fig. 6 from Nagata et al., 2017
nkhspGFF3AEt + MO1-kcnh6a		3 figures from Tanaka et al., 2018
nkhspGFF3AEt + MO1-mybpc3		2 figures from Hodatsu et al., 2014
nkhspGFF3AEt; zf3131Tg		Fig. 1 from Nagao et al., 2020	Fig. 1 from Nagao et al., 2020
nkhspGFF3AEt; zf3281Tg			Fig. 3 from Kioka et al., 2020
nkhspGFF53AEt
nkhspGFF53AEt; nkuasgfp1aTg			6 figures from 2 publications
nkhspGFF53AEt; nkuasgfp1aTg; rw0130aTg			Fig. 3 from Pujol-Martí et al., 2010
nkhspGFF53AEt; s1999tTg			2 figures from Zecca et al., 2015
nkhspGFF55BEt		Fig. 2 from Wai et al., 2015	Fig. 2 from Wai et al., 2015
nkhspGFF55BEt; nkuasgfp1aTg			Fig. 2 from Wai et al., 2015
nkhspGFF57AEt; nkuasgfp1aTg			Fig. 2 from Takeuchi et al., 2015
nkhspGFF62ATg
nkhspGFF62ATg; p201Tg			Fig. 1 from Bremer et al., 2019
nkhspGFF62ATg; p411Tg; ue2Tg			2 figures from Bremer et al., 2019
nkhspGFF62ATg; p411Tg; zf167Tg			2 figures from Bremer et al., 2019
nkhspGFF62ATg; y362Tg
nkhspGFF62ATg; y362Tg; zf206Et		Fig 5 from Meserve et al., 2021

Sequence Information

Other Construct Pages

None

Citations