FIGURE

Fig 5

ID

ZDB-FIG-210623-18

Publication

Meserve et al., 2021 - A forward genetic screen identifies Dolk as a regulator of startle magnitude through the potassium channel subunit Kv1.1

Other Figures

All Figure Page

Back to All Figure Page

Fig 5

Kv1.1 functions outside the Mauthner command neuron to control swim movement magnitude.

(A-D) kcna1a mutants expressing Tol056 (GFP in Mauthner cells; green), 62a:Gal4 (Mauthner specific) and UAS:NTR-RFP (magenta). 62a>NTR-RFP is mosaic so some fish do not display expression in Mauthner cells (A) while others do (B). Before treatment with metronidazole (Mtz) at 4 dpf, all fish have GFP+ Mauthner cells (A,B). After 24 hr Mtz treatment, Mauthner cells are present in fish without initial NTR-RFP Mauthner expression (C) and absent in fish that displayed initial Mauthner NTR-RFP expression (D). (E-H) Kinematic parameters of the acoustic startle response in kcna1a sibling and mutant larvae with or without NTR-RFP expression in Mauthners and with or without Mtz treatment. Each point is the average response over ten trials for an individual larva. n≥9 larvae, **p<0.0001 (one-way ANOVA with Tukey correction for multiple comparisons). Scale bars = 50 μM.

Expression Data

Genes:	EGFP TagRFPT
Fish:	kcna1a^p181/p181; nkhspGFF62ATg; y362Tg; zf206Et
Condition:	chemical ablation: Mauthner neuron, chemical treatment by environment: metronidazole
Anatomical Terms:	hindbrain Mauthner neuron
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkhspGFF62ATg; y362Tg; zf206Et kcna1a^p181/p181; nkhspGFF62ATg; y362Tg; zf206Et
Condition:	chemical ablation: Mauthner neuron, chemical treatment by environment: metronidazole
Observed In:	hindbrain Mauthner neuron startle response
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.