Delaying gal4-driven gene expression in the zebrafish with morpholinos and gal80
- Faucherre, A., and López-Schier, H.
- PLoS One 6(1): e16587 (Journal)
- Registered Authors
- Faucherre, Adele, Lopez-Schier, Hernan
- MeSH Terms
- Animals, Genetically Modified*
- DNA-Binding Proteins/genetics*
- Gene Expression Regulation, Developmental
- Repressor Proteins/genetics*
- Transcription Factors/genetics*
- Zebrafish Proteins/genetics*
- 21298067 Full text @ PLoS One
Faucherre, A., and López-Schier, H. (2011) Delaying gal4-driven gene expression in the zebrafish with morpholinos and gal80. PLoS One. 6(1):e16587.
The modular Gal4/UAS gene expression system has become an indispensable tool in modern biology. Several large-scale gene- and enhancer-trap screens in the zebrafish have generated hundreds of transgenic lines expressing Gal4 in unique patterns. However, the early embryonic expression of the Gal4 severely limits their use for studies on regeneration or behavior because UAS-driven effectors could disrupt normal organogenesis. To overcome this limitation, we explored the use of the Gal4 repressor Gal80 in transient assays and with stable transgenes to temporally control Gal4 activity. We also validated a strategy to delay Gal4-driven gene expression using a morpholino targeted to Gal4. The first approach is limited to transgenes expressing the native Gal4. The morphant approach can also be applied to transgenic lines expressing the Gal4-VP16 fusion protein. It promises to become a standard approach to delay Gal4-driven transgene expression and enhance the genetic toolkit for the zebrafish.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes