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ZFIN ID: ZDB-FIG-110318-27
Faucherre et al., 2011 - Delaying gal4-driven gene expression in the zebrafish with morpholinos and gal80. PLoS One   6(1):e16587 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Days 7-13

Fig. 6 Gal4 morpholino allows bypassing of early developmental stages.

(A–F) Embryos resulting from an incross between Tg[ET(hsp:Gal4VP16s1001t);UAS:Kaede] double trangenics (A,D) or from Tg[ET(hsp:Gal4VP16s1001t);UAS:Kaede] with Tg[UAS:NICD-Myc] animals (B–C, E–F) were either non-injected (NI, A–B, D–E) or injected with 3 ng of Gal4 MO (C,F). Representative specimens are depicted at 3 dpf. The lower panel shows a magnification of the different specimens after DiASP treatment (D–F). (G–J) Embryos resulting from a cross between Tg[ET(hsp:Gal4VP16s1006t)] and Tg[UAS:NICD-Myc] animals were either non-injected (G and magnification in H) or injected with 3 ng of Gal4 MO (I and magnification in J). Representative specimens are depicted at 6 dpf. (K–P) Embryos resulting from a cross between Tg[hspGFF53A;UAS:EGFP] double transgenic animals and either wt (K, N) or Tg[UAS:NICD-Myc] (L–M, O–P) animals were either non-injected (K–L, N–O) or injected with 3 ng of Gal4 MO (M, P). Representative specimens are depicted at 7 dpf. (K–M) Maximal projections of posterior lateralis afferent ganglion (dashed circles) and central projection (white arrowheads). (N–P) Maximal projections of posterior lateralis afferent nerve. (Scale bars: 150 μm for A–F, H and J; 300 μm for G and I; 100 μm for K–P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kca3Tg; nkhspGFF53AEt; nkuasgfp1aTg heat shock Days 7-13 posterior lateral line ganglion afferent neuron IFL
nkhspGFF53AEt; nkuasgfp1aTg heat shock Days 7-13 posterior lateral line ganglion afferent neuron IFL
Kaede kca3Tg; s1001tEt; s1999tTg heat shock Protruding-mouth neuromast IFL
Protruding-mouth skeletal muscle IFL
s1001tEt; s1999tTg heat shock Protruding-mouth neuromast IFL
Protruding-mouth skeletal muscle IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; nkhspGFF53AEt; nkuasgfp1aTg heat shock Days 7-13 afferent neuron neuron projection decreased amount, abnormal
Days 7-13 posterior lateral line ganglion decreased size, abnormal
kca3Tg; s1001tEt; s1999tTg heat shock Protruding-mouth heart edematous, abnormal
Protruding-mouth yolk inflated, abnormal
kca3Tg; s1006tEt heat shock Day 6 eye decreased size, abnormal
Day 6 head decreased size, abnormal
Day 6 heart edematous, abnormal
Day 6 whole organism decreased size, abnormal
Day 6 yolk inflated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One