PUBLICATION
Higd1a improves respiratory function in the models of mitochondrial disorder
- Authors
- Nagao, T., Shintani, Y., Hayashi, T., Kioka, H., Kato, H., Nishida, Y., Yamazaki, S., Tsukamoto, O., Yashirogi, S., Yazawa, I., Asano, Y., Shinzawa-Itoh, K., Imamura, H., Suzuki, T., Suzuki, T., Goto, Y.I., Takashima, S.
- ID
- ZDB-PUB-200110-2
- Date
- 2020
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34: 1859-1871 (Journal)
- Registered Authors
- Shintani, Yasunori
- Keywords
- cytochrome c oxidase, hypoxia, mitochondria, respiratory chain
- MeSH Terms
-
- Adenosine Triphosphate/metabolism
- Animals
- Animals, Genetically Modified
- Biological Transport/physiology
- Cell Line
- Cytochromes c/metabolism
- Electron Transport/physiology*
- Electron Transport Complex IV/metabolism
- HEK293 Cells
- Humans
- Hypoxia/metabolism
- Intracellular Signaling Peptides and Proteins/metabolism*
- Kinetics
- Mitochondria/metabolism*
- Mitochondrial Diseases/metabolism*
- Mitochondrial Proteins/metabolism*
- Oxidation-Reduction
- Respiration
- Zebrafish/metabolism
- PubMed
- 31914602 Full text @ FASEB J.
Citation
Nagao, T., Shintani, Y., Hayashi, T., Kioka, H., Kato, H., Nishida, Y., Yamazaki, S., Tsukamoto, O., Yashirogi, S., Yazawa, I., Asano, Y., Shinzawa-Itoh, K., Imamura, H., Suzuki, T., Suzuki, T., Goto, Y.I., Takashima, S. (2020) Higd1a improves respiratory function in the models of mitochondrial disorder. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 34:1859-1871.
Abstract
The respiratory chain (RC) transports electrons to form a proton motive force that is required for ATP synthesis in the mitochondria. RC disorders cause mitochondrial diseases that have few effective treatments; therefore, novel therapeutic strategies are critically needed. We previously identified Higd1a as a positive regulator of cytochrome c oxidase (CcO) in the RC. Here, we test that Higd1a has a beneficial effect by increasing CcO activity in the models of mitochondrial dysfunction. We first demonstrated the tissue-protective effects of Higd1a via in situ measurement of mitochondrial ATP concentrations ([ATP]mito) in a zebrafish hypoxia model. Heart-specific Higd1a overexpression mitigated the decline in [ATP]mito under hypoxia and preserved cardiac function in zebrafish. Based on the in vivo results, we examined the effects of exogenous HIGD1A on three cellular models of mitochondrial disease; notably, HIGD1A improved respiratory function that was coupled with increased ATP synthesis and demonstrated cellular protection in all three models. Finally, enzyme kinetic analysis revealed that Higd1a significantly increased the maximal velocity of the reaction between CcO and cytochrome c without changing the affinity between them, indicating that Higd1a is a positive modulator of CcO. These results corroborate that Higd1a, or its mimic, provides therapeutic options for the treatment of mitochondrial diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping