PUBLICATION

Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis

Authors
Dow, E., Siletti, K., Hudspeth, A.J.
ID
ZDB-PUB-150523-13
Date
2015
Source
Genes & Development   29: 1087-94 (Journal)
Registered Authors
Hudspeth, A.J. (Jim)
Keywords
auditory system, filopodium, lateral line, ribbon synapse, vestibular system, zebrafish
MeSH Terms
  • Cell Differentiation*
  • Lateral Line System/cytology*
  • Lateral Line System/growth & development
  • Lateral Line System/ultrastructure
  • Sensory Receptor Cells/cytology*
  • Sensory Receptor Cells/ultrastructure
  • Animals
  • Zebrafish/growth & development
  • Zebrafish/physiology*
  • Microscopy, Electron, Transmission
  • Synapses/physiology*
(all 11)
PubMed
25995190 Full text @ Genes & Dev.
Abstract
The assembly of a nervous system requires the extension of axons and dendrites to specific regions where they are matched with appropriate synaptic targets. Although the cues that guide long-range outgrowth have been characterized extensively, additional mechanisms are required to explain short-range guidance in neural development. Using a complementary combination of time-lapse imaging by fluorescence confocal microscopy and serial block-face electron microscopy, we identified a novel type of presynaptic projection that participates in the assembly of the vertebrate nervous system. Synapse formation by each hair cell of the zebrafish's lateral line occurs during a particular interval after the cell's birth. During the same period, projections emerge from the cellular soma, extending toward a specific subpopulation of mature hair cells and interacting with polarity-specific afferent nerve terminals. The terminals then extend along the projections to reach appropriately matched presynaptic sites, after which the projections recede. Our results suggest that presynaptic projections act as transient scaffolds for short-range partner matching, a mechanism that may occur elsewhere in the nervous system.
Genes / Markers
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    cwr2TgTransgenic Insertion
      knu3TgTransgenic Insertion
        nkhsp70GFF4AEtTransgenic Insertion
          ru1010TgTransgenic Insertion
            sqet4EtTransgenic Insertion
              vo8TgTransgenic Insertion
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                Human Disease / Model
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                Fish
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                Orthology
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                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                mCherryEFGmCherry
                NTREFGNTR
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