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Fig. 1

PG1 hox function is not required for expression of most r4 genes. Expression of r4 genes was assayed via ISH in (i) WT, (ii) hoxb1b mutant, (iii) hoxb1a mutant, (iv) hnf1ba mutant and (v) valentino mutant zebrafish. The genes assayed include a hoxb1a, b dusp2, c dusp6, d spry1, e fgf3, f fgf8, g efnb2a, h meis1a, i ccnjl, j irx7, k sall4, l greb1l, m egfl6, n hoxb2a and o engrailed1b. krox20 (red) which is expressed in r3 and r5, was used to assign the expression domains of several genes, as indicated. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 12hpf, 14hpf, 16hpf and 19hpf were imaged as whole-mounts. 24hpf embryos were flat-mounted for imaging. Black arrows point to the r4 domain in 12hpf and 24hpf embryos. White brackets mark the normal and expanded r4 domains in embryos staged at 14hpf, 16hpf and 19 hpf

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