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ZFIN ID: ZDB-FIG-171227-29
Eve et al., 2017 - Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes. PLoS One   12:e0172227 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 5

Cardiovascular associated genes are downregulated in tmem88aΔ8 embryos.

(A) qRT-PCR showing the expression of key cardiovascular genes in tmem88Δ8 mutants compared to Tg(fli1a:egfp) controls at 13 hpf. (B) In situ hybridisation for fli1a, gata1a, and myoD in control and tmem88aΔ8 mutants as labelled. Gata1a expression is denoted with black error heads, reduced or absent expression is shown with grey or white arrowheads respectively. Somites are marked by asterisks. (C) qRT-PCR showing the expression of key cardiovascular genes in tmem88Δ8 mutants compared to Tg(fli1a:egfp) controls at 48 hpf. All P-values determined by Unpaired Student’s t test. Error bars denote the standard deviation of three biological replicates. P-values determined by Unpaired Students t test. * = p<0.05; ** = p<0.01; *** = p<0.001; unlabelled bars show no significant difference (p>0.05). fli1a, friend leukaemia integration 1a; gata1a, GATA-binding factor 1a; hbbe1, haemoglobin beta-embryonic 1; hpf, hours post fertilisation; lyz, lysozyme; runx1, runt-related transcription factor 1; scl, stem cell leukaemia; spi1, spleen focus forming virus (sffv) and proviral integration oncogene 1b (also known as pu.1).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fli1a y1Tg standard conditions 5-9 somites whole organism RTPCR
10-13 somites heart primordium ISH
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
10-13 somites heart primordium ISH
gata1a y1Tg standard conditions 5-9 somites whole organism RTPCR
10-13 somites angioblastic mesenchymal cell ISH
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
10-13 somites angioblastic mesenchymal cell ISH
hbbe1.1 y1Tg standard conditions 5-9 somites whole organism RTPCR
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
myod1 y1Tg standard conditions 10-13 somites somite ISH
y1Tg ; tmem88afci400/fci400 standard conditions 10-13 somites somite ISH
runx1 y1Tg standard conditions 5-9 somites whole organism RTPCR
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
spi1b y1Tg standard conditions 5-9 somites whole organism RTPCR
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
tal1 y1Tg standard conditions 5-9 somites whole organism RTPCR
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism hbbe1.1 expression decreased amount, abnormal
5-9 somites whole organism spi1b expression decreased amount, abnormal
5-9 somites whole organism gata1a expression decreased amount, abnormal
10-13 somites angioblastic mesenchymal cell gata1a expression decreased amount, abnormal
10-13 somites somite myod1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One