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ZFIN ID: ZDB-FIG-171227-31
Eve et al., 2017 - Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes. PLoS One   12:e0172227 Full text @ PLoS One
Anatomical Term:
Stage Range: 5-9 somites to Long-pec
Observed In:
Stage: Long-pec

Fig. 7

Erythropoiesis was not affected in tmem88aΔ8 zebrafish mutants.

(A-D) o-Dianisidine staining of erythrocytes at 48 hpf in uninjected Tg(fli1a:egfp) controls (A, n = 16) or controls injected with tmem88a SBMO (B, n = 12), and uninjected tmem88aΔ8 mutants (C, n = 18) or mutants injected with tmem88a SBMO (D, n = 15). (E) The area of o-Dianisine staining was quantified and presented as a percentage of the total yolk area, shown in a Tukey box and whisker plot. No significant difference was found between control embryos and tmem88aΔ8 mutants (p = 0.2710, Unpaired Student’s t test). (F) tmem88b expression is not affected in tmem88aΔ8 mutants. qRT-PCR showing the expression of tmem88b in tmem88Δ8 mutants compared to Tg(fli1a:egfp) controls at 13 and 48 hpf. P-value determined by Unpaired Student’s t test. Error bars denote the standard deviation of three biological replicates. A, anterior; h, hours post fertilisation; D, dorsal; L, lateral; V, ventral; n.s., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tmem88b y1Tg standard conditions 5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg ; tmem88afci400/fci400 standard conditions Long-pec nucleate erythrocyte amount, normal
Long-pec primitive erythrocyte differentiation process quality, normal
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One