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ZFIN ID: ZDB-FIG-171227-31
Eve et al., 2017 - Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes. PLoS One   12:e0172227 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 7

Erythropoiesis was not affected in tmem88aΔ8 zebrafish mutants.

(A-D) o-Dianisidine staining of erythrocytes at 48 hpf in uninjected Tg(fli1a:egfp) controls (A, n = 16) or controls injected with tmem88a SBMO (B, n = 12), and uninjected tmem88aΔ8 mutants (C, n = 18) or mutants injected with tmem88a SBMO (D, n = 15). (E) The area of o-Dianisine staining was quantified and presented as a percentage of the total yolk area, shown in a Tukey box and whisker plot. No significant difference was found between control embryos and tmem88aΔ8 mutants (p = 0.2710, Unpaired Student’s t test). (F) tmem88b expression is not affected in tmem88aΔ8 mutants. qRT-PCR showing the expression of tmem88b in tmem88Δ8 mutants compared to Tg(fli1a:egfp) controls at 13 and 48 hpf. P-value determined by Unpaired Student’s t test. Error bars denote the standard deviation of three biological replicates. A, anterior; h, hours post fertilisation; D, dorsal; L, lateral; V, ventral; n.s., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tmem88b y1Tg standard conditions 5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
y1Tg ; tmem88afci400/fci400 standard conditions 5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg ; tmem88afci400/fci400 standard conditions Long-pec nucleate erythrocyte amount, normal
Long-pec primitive erythrocyte differentiation process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One