ZFIN ID: ZDB-FIG-170321-25
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Fig. 3

MZstat3 mutants exhibit transient and mild extension defects in axial mesoderm during gastrulation.

(A) Live images of WT and MZstat3 embryos shown in lateral (a, c) and dorsal view (b). The insets in c show the part of notochord above yolk extension in 30 hpf embryos. Arrowhead, nt, notochord. (B) Morphometric analysis of AP axis extension of 30 hpf embryos shown in A(c). (C) papc in presomitic mesoderm and dlx3b marking neuroectoderm boundary in 1-somite stage WT and MZstat3 embryos (dorsal view). (D) Measurement of ML width of papc expression domain (pink in C). (E) Expression of ntl in notochord and tail in 1-somite stage WT, Zstat3, Mstat3, MZstat3, and MZstat3 embryos overexpressing Stat3-F, as well as WT and MZstat3 embryos injected with 5 ng MO1-stat3 (lateral view). Phenol red was used as injection control. (F) Measurement of notochord length in embryos in E (blue lines in E). ****p<0.0001, n.s. = non-significant, error bars = SEM. See also S3 Fig.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT standard conditions 1-4 somites neuroectoderm ISH
stat3stl27/stl27 standard conditions 1-4 somites neuroectoderm ISH
pcdh8 WT standard conditions 1-4 somites segmental plate ISH
stat3stl27/stl27 standard conditions 1-4 somites segmental plate ISH
tbxta WT standard conditions 1-4 somites notochord ISH
WT + MO1-stat3 standard conditions 1-4 somites notochord ISH
stat3stl27/stl27 standard conditions 1-4 somites notochord ISH
stat3stl27/stl27 standard conditions 1-4 somites notochord ISH
stat3stl27/stl27 + MO1-stat3 standard conditions 1-4 somites notochord ISH
stat3stl27 standard conditions 1-4 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-stat3 standard conditions 1-4 somites notochord decreased length, abnormal
stat3stl27/stl27 standard conditions 1-4 somites notochord decreased length, abnormal
stat3stl27/stl27 + MO1-stat3 standard conditions 1-4 somites notochord decreased length, exacerbated
stat3stl27 standard conditions 1-4 somites notochord decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.