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ZFIN ID: ZDB-FIG-170321-27
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Observed In:
Stage Range: 32-cell to Bud

Fig. 5

Stat3 promotes cell cycle progression during zebrafish embryogenesis.

(A) Immunofluorescent anti-pH3 labeling of proliferating cells (red) and DAPI labeling all nuclei (blue) in WT, Mstat3, and MZstat3 embryos at 6 hpf (animal view) and 10 hpf (dorsal view). (B, D) Quantification of mitotic cell number at 6 and 10 hpf. (C, E) Quantification of total cell number at 6 and 10 hpf. (F and G) Average length of each cell cycle (F) and timing of mitosis (G) from Cycle 5 to Cycle 9 in embryos from WT, stat3stl27/+, and stat3stl27/+ females. (H-K) Analyses of cell divisions from Cycle 5 to Cycle 9 in 10 pg (H and I) and 25 pg (J and K) stat3-F injected MZstat3 embryos. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, n.s. = non-significant, error bars = SEM. See also S5 Fig.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-h3 IHC WT standard conditions Shield whole organism cell
IHC Bud whole organism cell
IHC stat3stl27/stl27 standard conditions Shield whole organism cell
IHC Bud whole organism cell
IHC stat3stl27 standard conditions Shield whole organism cell
IHC Bud whole organism cell
Phenotype Details
Fish Conditions Stage Phenotype
stat3stl27/stl27 standard conditions Shield cell population proliferation decreased occurrence, abnormal
Shield mitotic cell cycle, embryonic decreased occurrence, abnormal
Shield whole organism cell decreased amount, abnormal
Shield whole organism cell ab4-h3 labeling decreased distribution, abnormal
Bud cell population proliferation decreased occurrence, abnormal
Bud mitotic cell cycle, embryonic decreased occurrence, abnormal
Bud whole organism cell decreased amount, abnormal
Bud whole organism cell ab4-h3 labeling decreased distribution, abnormal
stat3stl27 standard conditions 32-cell - 512-cell mitotic cell cycle increased duration, abnormal
Shield cell population proliferation decreased occurrence, abnormal
Shield mitotic cell cycle, embryonic decreased occurrence, abnormal
Shield whole organism cell decreased amount, abnormal
Shield whole organism cell ab4-h3 labeling decreased distribution, abnormal
Bud whole organism cell decreased amount, abnormal
Bud whole organism cell ab4-h3 labeling decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.