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ZIRC
ZFIN ID: ZDB-FIG-170321-24
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-cell to Days 45-89

Fig. 2

Zebrafish stat3 mutants develop late-onset scoliosis and cannot survive to adulthood.

(A) Growth curve of stat3stl28/stl28 fish and siblings. (B) Body length of stat3stl27/stl27 and stat3stl27/+ and WT siblings at 18 dpf. Different genotypes were kept in the same tank and fed exclusively with rotifers to diminish food competition. (C) Survival rate of stat3stl27/stl27, stat3stl27/+ fish and WT siblings. (D) Images of live animals and Alizarin stained skeletons showing WT and zygotic stat3 mutant fish at 15 dpf (anterior to the left). Vertebral structures in the boxed region were revealed via Alizarin Red live staining and confocal imaging, shown in insets. (E) Images of live animals and Alizarin stained skeletons showing a WT and a zygotic stat3stl28/stl28 mutant fish with two curves near the caudal fin (inset) at 22 dpf. Anterior to the left. (F) Images of live animals and Alizarin stained skeletons showing a WT and a scoliotic stat3stl27/stl27 mutant fish at 46 dpf (anterior to the left). (G) μCT imaging showing body curvatures of WT sibling and stat3stl27/stl27 mutant at 41 dpf (anterior to the left). (H) μCT imaging showing body curvatures of WT sibling and stat3sa15744/sa15744 mutant at 43 dpf (anterior to the left, arrow denote bend in spine). (I-J) Quantification of bone mineral density (H) and bone/tissue volume ratio (Bv/Tv, I) of stat3stl27/stl27 (G). (K-L) Transcript levels of tnfα and il6 in WT and stat3stl27/stl27 mutant animals detected by qRT-PCR at indicated developmental stages. (M-N) Survival rate and percentage of mutant fish with various numbers of body curves of MZ stat3stl27/stl27 mutants. *p<0.05, ***p<0.001, ****p<0.0001, error bars = SEM. See also S2 Fig.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
il6 stat3stl27/stl27 standard conditions Days 30-44 whole organism RTPCR
tnfa stat3stl27/stl27 standard conditions Days 30-44 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
stat3sa15744/sa15744 standard conditions Days 30-44 spinal cord bent, abnormal
Days 30-44 whole organism decreased length, abnormal
stat3stl27/stl27 standard conditions 1-cell - Days 45-89 spinal cord bent, abnormal
1-cell - Days 45-89 whole organism decreased life span, abnormal
stat3stl27/stl27 standard conditions Days 14-20 whole organism decreased length, abnormal
Days 14-20 - Days 45-89 whole organism decreased life span, abnormal
stat3stl27/stl27 standard conditions Days 30-44 spinal cord bent, abnormal
Days 30-44 whole organism decreased length, abnormal
stat3stl27/stl27 standard conditions Days 30-44 whole organism il6 expression increased amount, abnormal
Days 30-44 whole organism tnfa expression increased amount, abnormal
stat3stl27/stl27 standard conditions Days 45-89 bone mineralization decreased occurrence, abnormal
Days 45-89 bone tissue decreased volume, abnormal
Days 45-89 spinal cord bent, abnormal
Days 45-89 whole organism decreased length, abnormal
stat3stl28/stl28 standard conditions Days 21-29 whole organism decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.