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ZFIN ID: ZDB-FIG-170321-31
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 16-cell to 1-4 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 16-cell to 1-4 somites

Fig. 9

Stat3 promotes cell cycle progression during zebrafish embryogenesis via upregulation of cdc25a expression.

(A) qRT-PCT analysis of stat3 and cdc25a transcript levels at 1.5 hpf. All results shown were normalized to gapdh. (B) qRT-PCR analysis of cdc25a transcript levels at 8.3 hpf. (C and D) Pre-MBT (C) and post-MBT (D) cell cycle length analyses in 1-somite stage MZstat3 embryos injected with 25 or 50 pg cdc25a RNA. Phenol Red was used as injection control. (E and F) ntl expression in 1-somite stage MZstat3 and WT gastrulae misexpressing Cdc25a (lateral view, anterior to the top). Notochord length (blue line) was quantified in F. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, error bars = SEM. See also S9 Fig.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdc25b stat3stl27/stl27 standard conditions 16-cell whole organism RTPCR
75%-epiboly whole organism RTPCR
stat3 stat3stl27/stl27 standard conditions 16-cell whole organism RTPCR
tbxta WT standard conditions 1-4 somites notochord ISH
stat3stl27/stl27 standard conditions 1-4 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
stat3stl27/stl27 standard conditions 16-cell whole organism stat3 expression decreased amount, abnormal
16-cell whole organism cdc25b expression decreased amount, abnormal
75%-epiboly whole organism cdc25b expression decreased amount, abnormal
1-4 somites notochord decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.