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ZFIN ID: ZDB-FIG-170321-28
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
Observed In:
Stage Range: High to Sphere

Fig. 6

Stat3 promotes post-MBT cell divisions but does not regulate cell division orientation during zebrafish embryogenesis.

(A) Experimental design for post-MBT cell cycle analyses. Embryos labeled ubiquitously with histone2B-RFP (H2B-RFP) were mosaically injected with memCherry or mGFP + stat3-F mRNA at 8-cell stage for lineage tracing. Labeled clones (B and C) within the same embryo were monitored using confocal time-lapse microscopy (See also Materials and methods). (D) Analyses of cell cycle lengths for Cycle 11–13 in WT, WT overexpressing Stat3-F, MZstat3, and MZstat3 overexpressing Stat3-F embryos. (E-G) Cell division orientation in dorsal neuroectoderm in WT (E) and MZstat3 (F) embryos during 1–3 somite stages, with 0 degrees representing mediolaterally aligned cell divisions and 90 degrees representing anteroposteriorly aligned cell divisions, quantified in G. *p<0.05, **p<0.01, ****p<0.0001, n.s. = non-significant, error bars = SEM. See also S7 Fig.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
stat3stl27/stl27 standard conditions High - Sphere mitotic cell cycle increased duration, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.