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ZFIN ID: ZDB-FIG-170321-26
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to Protruding-mouth

Fig. 4

MZstat3 mutants show neither obvious cell polarity defects during C&E nor interaction with zebrafish PCP mutants.

(A) Confocal image of dorsal mesoderm in WT, Mstat3, and MZstat3 1-somite stage embryos labeled with mGFP (anterior to the top). Cell shape and orientation of notochord cells outlined in yellow were analyzed as illustrated in D. (B) Measurement of notochord width at 1-somite stage. (C) Quantification of the number of cells across the ML notochord axis at 1-somite stage. (E) Cumulative distribution of notochord cell orientation in WT, Mstat3, and MZstat3 embryos. (F) Cell shape analysis represented by length-to-width ratio (LWR). (G-I) Long axis (length, G), short axis (width, H) and average area (I) of in WT, Mstat3 and MZstat3 notochord cells. (J) A spectrum of eye separation phenotypes at 3 dpf with C1 representing WT eye spacing and C5 representing the most severe phenotype, cyclopia. Ventral view, anterior to the top. (K) Penetrance and expressivity of eye separation phenotypes of Ztri, Ztri;Zstat3stl27/+, and Ztri;Zstat3 embryos. Eye separation phenotypes were also quantified by cyclopia index (CI) as previously described [36].(L) Penetrance and expressivity of eye separation phenotypes of MZslb, MZslb;Zstat3stl27/+, and MZslb;Zstat3 embryos. ****p<0.0001, n.s. = non-significant, error bars = SEM. See also S4 Fig.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
stat3stl27/stl27 standard conditions 1-4 somites notochord cell decreased length, abnormal
1-4 somites notochord cell increased area, abnormal
1-4 somites notochord cell increased width, abnormal
1-4 somites notochord cell shape, abnormal
stat3stl27/stl27 ; vangl2vu67/vu67 standard conditions Protruding-mouth eye adjacent to eye, abnormal
Protruding-mouth eye fused with eye, abnormal
stat3stl27 standard conditions 1-4 somites notochord cell decreased length, abnormal
1-4 somites notochord cell increased area, abnormal
1-4 somites notochord cell increased width, abnormal
1-4 somites notochord cell shape, abnormal
vangl2vu67/vu67 standard conditions Protruding-mouth eye adjacent to eye, abnormal
Protruding-mouth eye fused with eye, abnormal
vangl2vu67/vu67 ; stat3stl27/+ standard conditions Protruding-mouth eye adjacent to eye, abnormal
Protruding-mouth eye fused with eye, abnormal
wnt11f2tz216/tz216 standard conditions Protruding-mouth eye adjacent to eye, abnormal
Protruding-mouth eye fused with eye, abnormal
wnt11f2tz216/tz216; stat3stl27/+ standard conditions Protruding-mouth eye adjacent to eye, abnormal
Protruding-mouth eye fused with eye, abnormal
wnt11f2tz216/tz216; stat3stl27/stl27 standard conditions Protruding-mouth eye adjacent to eye, abnormal
Protruding-mouth eye fused with eye, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.