ZFIN ID: ZDB-FIG-140328-18
Lister et al., 2014 - A Conditional Zebrafish MITF Mutation Reveals MITF Levels Are Critical for Melanoma Promotion vs. Regression In Vivo. The Journal of investigative dermatology   134(1):133-40 Full text @ J. Invest. Dermatol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 3

MITF target gene expression. (a–i) Box plots of quantitative real-time PCR (qRT-PCR) of MITF target genes and p53. The y-axis indicates the difference between the cycle threshold (Ct) value of the gene of interest and the Ct value of β-actin in each sample. Note that the y-axis is inverted for ease of interpretation. Bars represent interquartile range; P-values determined by Student’s t-test. Also see Supplementary Table S1 online. MITF, microphtholmia associated transcription factor.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bcl2a mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
cdk2 mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
cdkn1a mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
cdkn2a/b mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
dct mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
hif1ab mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
met mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
tp53 mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
tyr mitfavc7/vc7; czt13Tg/czt13Tg cold shock Adult whole organism RTPCR
tp53zdf1/zdf1; czt13Tg standard conditions Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mitfavc7/vc7; czt13Tg/czt13Tg heat shock Adult whole organism melanoma decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The Journal of investigative dermatology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Invest. Dermatol.